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anionic polyacrylamide gel protocol in united kingdom

  • what is cationic polyacrylamide gel in united kingdom

    Polyacrylamide polymer msds Polyacrylamide is a kind of linear polymers,it can soluble in water,insoluble in most organic solvents. According to the product features can be divided into:anionic polyacrylamide,cationic polyacrylamide,and non-ionic polyacrylamide. it is widely used in oil recovery,mineral processing,coal … Get Price

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  • SDS Polyacrylamide Gel Electrophoresis of Proteins ...

    Abstract. Probably the most widely used technique for analyzing mixtures of proteins is SDS polyacrylamide gel electrophoresis. In this technique, proteins are reacted with the anionic detergent, sodium dodecylsulfate (SDS, or sodium lauryl sulfate) to form negatively charged complexes. The amount of SDS bound by a protein, and so the charge on ...

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  • Polyacrylamide Gel Electrophoresis - CSH Protocols

    Polyacrylamide gels have the following three major advantages over agarose gels: (1) Their resolving power is so great that they can separate molecules of DNA whose lengths differ by as little as 0.1% (i.e., 1 bp in 1000 bp). (2) They can accommodate much larger quantities of DNA than agarose gels. Up to 10 µg of DNA can be applied to a single ...

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  • Separating Protein: SDS-Polyacrylamide Gel

    There are two common types of gel: polyacrylamide and agarose. For most applications, denaturing acrylamide gels are most appropriate. These gels are extremely versatile and can resolve RNAs from ~600 to ≤20 nucleotides (nt). In certain circumstances, e.g., resolving different conformers of RNAs or RNA-protein complexes, native gels are ...

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  • Polyacrylamide Gel Electrophoresis of RNA

    2018-4-20 · Markers using a Non-denaturing Polyacrylamide Gel Ling Huang*1, Xiaohui Deng*1, Ronghua Li1, Yanshi Xia1, Guihua Bai2, Kadambot H.M. Siddique3, Peiguo Guo1 1College of Life Sciences, Guangzhou University 2Hard Winter Wheat Genetics Research Unit, United States Department of Agriculture - Agricultural Research Service

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  • Markers using a Non-denaturing Polyacrylamide Gel A Fast ...

    The original two-dimensional gel electrophoresis format was developed almost 30 years ago to exploit this variation in protein charge and size for separation purposes. The isoelectric point of a protein (pI) is the pH at which it has a net zero charge, which, for the majority of proteins, lies between pH 4.0 and 8.0.

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  • Large-Format 2-D Polyacrylamide Gel Electrophoresis ...

    2017-9-24 · Accepta 4364 is a high performance; superior quality solid form anionic polymer – 50% Active. Accepta 4364 is available in a convenient block form making it easy to use, store and handle. Also known as a flocc-block it has been specially developed to slowly dissolve directly into the effluent flow in question and flocculate the associated suspended solids.

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  • Anionic Polymer Blocks - Flocc Block for remote industrial ...

    1997-12-15 · Author information: (1)Department of Biological Sciences, University of Essex, Colchester, United Kingdom. An anionic chymotrypsin-like enzyme was isolated from a crude extract of camel pancreas by a three-step procedure consisting of anion-exchange chromatography, gel filtration, and hydrophobic interaction chromatography.

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  • Purification and partial characterization of camel anionic ...

    2020-7-11 · The hydrated PAM is a soft gel that is used in gel electrophoresis and as a super water-absorbing polymer (SAP's). Even though these polymers are called polyacrylamide, they are often copolymers of acrylamide and one or more other monomers. The most important co-monomer is acrylic acid or sodiumacrylate.

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  • Polyacrylamide Gel Staining Protocols - ResearchGate

    Polyacrylamide Gel Staining Protocols ... Gel staining protocol. a. Fix gel in fixing solution for about 30 mins. b. Stain gel in coomassie working solution for about 25-40 minutes.

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  • Polyacrylamide Gel Electrophoresis | Cleaver Scientific

    2007-10-25 · This protocol describes a simple silver staining method used to visualize DNA fragments and other organic molecules with unsurpassed detail following traditional polyacrylamide gel electrophoresis ...

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  • Silver staining DNA in polyacrylamide gels | Nature

    Transfer Protocol: Load the protein sample onto a 4-20% Tris-Glycine polyacrylamide gel and run until desired resolution is achieved. (The electrophoresis can be followed by using pre-stained molecular weight markers). Set up the Submerged Western Transfer Cassette as described below and in Figure III-2:

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  • Protocols - Western Transfer (Western Blot)

    2019-2-5 · polyacrylamide around gel. 19. Hold two pieces of dry 46 × 57–cm blotting paper together as one piece. Beginning at one end of gel and working slowly towards the other, lay paper on top of gel. Take care to prevent air bubbles from forming between paper and gel. 20. Peel blotting paper up; gel should come off plate with it. Gradually curl ...

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  • Denaturing Polyacrylamide Gel Electrophoresis

    Transfer Protocol: Load the protein sample onto a 4-20% Tris-Glycine polyacrylamide gel and run until desired resolution is achieved. (The electrophoresis can be followed by using pre-stained molecular weight markers). Set up the Submerged Western Transfer

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  • Protocols - Western Transfer (Western Blot)

    2021-5-22 · Tuning the Range of Polyacrylamide Gel Stiffness for Mechanobiology Applications Aleksandra K. DenisinTuning the mechanical properties of alginate–peptide hydrogels. Soft Matter 2018, 14 (21) , 4364-4373. DOI: 10.1039 Ilaria Sanzari, Mauro . Tuning the Range of Polyacrylamide Gel Stiffness for Mechanobiology Applications

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  • tuning the range of polyacrylamide gel stiffness ...

    After chemical synthesis, the oligonucleotide preparation contains the desired full-length oligonucleotide but also all of the DNA molecules that were aborted during each cycle in the synthesis, and the by-products generated during the chemical reactions. The purification of oligonucleotides is a cr …

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  • Purification of DNA Oligos by denaturing polyacrylamide ...

    Name:PAM Anionic Polyacrylamide 1.CAS NO 9003-05-8 HS Code 3906901000 2.EINECS No 201-173-7 3.MF [CH2=CHCONH2]n 4.appearance white crystal powder 5.Specification: Polyacriylamide Anionic PAM 5-8 million Molecular weight:5-8 million Solid content:Solid content Free monomer:0.025% max Dissolving time :1h max Water Insolubles:0.3% max Hydrolyzing …

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  • Pam Anionic Polyacrylamide by CN Sinosou Of Welcom

    2017-6-7 · Agarose Gel Protocol See long version for background DNA gels are used to separate fragments of DNA and RNA. Unlike most protein separations which use acrylamide polymers, use agarose in a submerged horizontal orientation, and at time called horizontal gel electrophoresis. This handout will cover the details of agarose gels, the theory of

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  • Introduction to Polyacrylamide Gels | LSR | Bio-Rad

    Polymerized acrylamide (polyacrylamide) forms a mesh-like matrix suitable for the separation of proteins of typical size. The strength of the gel allows easy handling. Polyacrylamide gel electrophoresis of SDS-treated proteins allows researchers to separate proteins based on their length in an easy, inexpensive, and relatively accurate manner.

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  • The principle and method of SDS-polyacrylamide gel ...

    Polyacrylamide gel electrophoresis (PAGE) analysis can be conveniently applied to analyze the molecular weight of sulfated GAGs. Gels on which GAGs have been fractionated can be visualized with Alcian Blue with or without silver staining and the bands can be scanned and digitized. The average MW of a GAG is then calculated based on a mixture of HP-derived oligosaccharide …

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  • Polyacrylamide Gel Electrophoresis - an overview ...

    Detection of DNA in Polyacrylamide Gels by Staining (Protocol summary only for purposes of this preview site) Unlike agarose gels, polyacrylamide gels cannot be cast in the presence of ethidium bromide because the dye inhibits polymerization of the acrylamide. However, ethidium bromide can be used to stain the polyacrylamide gel after ...

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  • Detection of DNA in Polyacrylamide Gels by Staining

    Do you have a protocol for polyacrylamide gel analysis of oligonucleotides? FAQ ID -961. Yes, please follow the Supplementary Protocol 'Polyacrylamide_gel_analysis_of_oligonucleotides' (PCR03).

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  • Do you have a protocol for polyacrylamide gel analysis of ...

    2018-9-7 · The hydrolyzed form of polyacrylamide (HPAM), a co-polymer of acrylamide and acrylic acid, is the most widely used anionic PAM in oil and gas development as well as in …

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  • Polyacrylamide Gel Electrophoresis (PAGE ...

    2018-5-1 · 6A. Quality Control Check and Size Selection using 6% Polyacrylamide Gel NEBNext Small RNA Library Prep Set for Illumina (E7300, E7580, E7560, E7330) This protocol follows Protocol for use with NEBNext Small RNA Library Prep …

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  • Polyacrylamide degradation and its implications in ...

    In-Gel Westerns directly detect protein in the polyacrylamide gel, without membrane transfer or blocking. Near-infrared (NIR) fluorescent In-Gel Westerns can be imaged with the Odyssey ® CLx or Classic Imagers when using IRDye ® secondary antibodies for detection. Figure 1. Multiplex detection of two target proteins by In-Gel Western.

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  • 6A. Quality Control Check and Size Selection using 6% ...

    1988-11-15 · ANALYTICAL BIOCHEMISTRY 175, 298-299 (1988) Alcian Blue Staining of Proteoglycans in Polyacrylamide Gels Using the 'Critical Electrolyte Concentration' Approach RITA S. WALL AND TRACY J. GYI The Open University, Oxford Research Unit, Boars Hill, Oxford OX15HR, United Kingdom Received June 17, 1988 A method is described here for Alcian blue staining of proteoglycans in polyacrylamide …

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  • Purificationof DNA using nondenaturing polyacrylamide gel ...

    2016-8-9 · Thebasic protocol describes the preparation of polyacrylamide gels for separationof small, double-stranded DNA fragments. After gel setup, DNA samples areloaded, electrophoresed through the gel, and finally purified away fromthe gel slices. Materials. 10xand 1x TBE electrophoresis buffer, pH 8.0 (APPENDIX 2)

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  • Polyacrylamide degradation and its implications in ...

    2018-9-7 · The hydrolyzed form of polyacrylamide (HPAM), a co-polymer of acrylamide and acrylic acid, is the most widely used anionic PAM in oil and gas development as well as in …

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  • BASIC PROTOCOL: PURIFICATION OF

    2016-8-9 · However, if the plates are particularly dirtyor if the complete removal of any residual nucleic acids is required, theplates may be soaked in an 0.1 M NaOH for 30 minutes prior to washing.If the gel is particularly thin (<1 mm), silanizing one or both of theplates facilitates post-electrophoretic separation of the gel from theplate.

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  • Running agarose and polyacrylamide gels

    2011-6-17 · The basics. Agarose gels can be used to resolve large fragments of DNA. Polyacrylamide gels are used to separate shorter nucleic acids, generally in the range of 1−1000 base pairs, based on the concentration used (Figure 1). These gels can be run with or without a denaturant. Gels that are run without a denaturant are referred to as native gels.

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  • Q4B Annex 10 Step 5 Polyacrylamide gel electrophoresis ...

    SDS-Polyacrylamide Gel Electrophoresis as it appears in the Japanese Pharmacopoeia Fifteenth Edition (March 31, 2006, The Ministry of Health, Labour and Welfare Ministerial Notification No. 285). 5.2.3 United States Pharmacopeia (USP): <1056> Biotechnology-derived Articles – Polyacrylamide Gel Electrophoresis official in USP 32, May 1, 2009.

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  • Human Cells using Blue Native Polyacrylamide Gel ...

    2021-5-7 · Protocol spaNOTE: This protocol is based on an associated publication by Hilander et al.13. 1. Preparation of buffers and solutions NOTE: All the buffers and solutions used in the protocol are summarized in Table 1. The given volumes of the buffers are sufficient to prepare and run 10 samples. All the buffers can be stored at +4 °C for up to ...

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  • Blue Native Polyacrylamide Gel Electrophoresis (BN‐PAGE ...

    2020-4-6 · Basic Protocol: . Oligomerization of proteins controls numerous biochemical features, such as the stability of proteins and the activity of enzymes, immune receptors, and ion channels (Gell, Grant, & Mackay, 2012).Gel filtration and blue native polyacrylamide gel electrophoresis (BN-PAGE) are the two principle approaches to studying native protein oligomerization in vitro and in …

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  • The principle and method of polyacrylamide gel ...

    Polymerized acrylamide (polyacrylamide) forms a mesh-like matrix suitable for the separation of proteins of typical size. The strength of the gel allows easy handling. Polyacrylamide gel electrophoresis of SDS-treated proteins allows researchers to separate proteins based on their length in an easy, inexpensive, and relatively accurate manner.

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  • Red Nucleic Acid Gel Stain (10,000×) - MedChemExpress

    2013-1-1 · Polyacrylamide gel purification (PAGE purification) is the method of choice when the highest percentage of full-length oligonucleotide is desired. This chapter describes a protocol for oligonucleotide purification using denaturing polyacrylamide gel electrophoresis, and includes oligonucleotide preparation, polyacrylamide gel electrophoresis ...

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  • Running agarose and polyacrylamide gels

    2011-6-17 · The basics. Agarose gels can be used to resolve large fragments of DNA. Polyacrylamide gels are used to separate shorter nucleic acids, generally in the range of 1−1000 base pairs, based on the concentration used (Figure 1). These gels can be run with or without a denaturant. Gels that are run without a denaturant are referred to as native gels.

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  • (PDF) A Guide to Polyacrylamide Gel Electrophoresis and ...

    For protein separation, virtually In gel electrophoresis, proteins do not all enter the all methods use polyacrylamide as an anticonvective, gel matrix at the same time. Samples are loaded sieving matrix covering a protein size range of into wells, and the proteins that are closer to the gel 5–250 kD.

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  • Do you have a protocol for polyacrylamide gel analysis of ...

    Do you have a protocol for polyacrylamide gel analysis of oligonucleotides? FAQ ID -961. Yes, please follow the Supplementary Protocol 'Polyacrylamide_gel_analysis_of_oligonucleotides' (PCR03).

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  • 10X SDS-PAGE Running Gel Buffer MB-017

    SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature. SDS is an anionic detergent applied to protein sample to linearize proteins and to impart a negative …

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  • Polyacrylamide Gel Electrophoresis (Theory) : Molecular ...

    The gel used is divided into an upper 'stacking' gel of low percentage (with large pore size) and low pH (6.8), where the protein bands get squeezed down as a thin layer migrating toward the anode and a resolving gel (pH 8.8) with smaller pores. Cl-is the only mobile anion present in both gels. When electrophoresis begins, glycine present in ...

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  • SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL

    2021-3-4 · Once the gel has polymerized it can be wrapped in gladwrap and stored at least for several days at 4 OC. 7. To continue, remove ethanol and dry in between the glass plates with filter paper. 8. Make monomer solution for the stacking gel by mixing the following reagents (enough for 2 gels). Percentage Acrylamide/Bis in gel 4,5 % Water (ml) 5,9

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  • Polyacrylamide Market Led by Anionic Polyacrylamide to ...

    2016-11-24 · The polyacrylamide market is estimated to reach USD 3.85 billion by 2021, at a CAGR of 6.8% from 2016 to 2021. In recent years, the Enhance Oil Recovery (EOR) technology is being rapidly adopted ...

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  • Blue native polyacrylamide gel electrophoresis for the ...

    2020-5-11 · This protocol will be helpful for new researchers conducting related experiments to analyze stable protein interactions including homo‐ or hetero‐oligomerization in plants. Ayutthaya PPN, Lundberg D, Weigel D, Li L. Blue native polyacrylamide gel electrophoresis (BN‐PAGE) for the analysis of protein oligomers in plants.

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  • Early Stage Equine OA: Vets Consider 2.5% Polyacrylamide ...

    Protocol - Elution of miRNA from the Gel Slice Recover the gel slice that contains miRNA, add 10 ml 1 M NaCl, crush gel, and incubate overnight at 4°C with rocking Excise a band from the gel starting just below the center of the xylene cyanol band, and extending to three quarters of the way to the bromophenol blue band.

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  • Polyacrylamide gel electrophoresis protocol pdf

    2021-4-6 · polyacrylamide gel electrophoresis (SDS‐PAGE) is a technique used to move charged molecules through a gel matrix by means of an electric current. This procedure is used to determine protein subunit composition, verify homogeneity of the protein sample, and purify proteins for use in other applications.

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  • anionic polyacrylamide gel - aladine.be

    Polyacrylamide Gel Electrophoresis (PAGE. 2020-01-14· SDS-PAGE (Polyacrylamide Gel Electrophoresis), is an analytical method used to separate components of a protein mixture based on their size. The technique is based upon the principle that a charged molecule will migrate in an electric field towards an electrode with opposite sign.

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  • Do you have a protocol for polyacrylamide gel analysis of ...

    Do you have a protocol for polyacrylamide gel analysis of oligonucleotides? FAQ ID -961. Yes, please follow the Supplementary Protocol 'Polyacrylamide_gel_analysis_of_oligonucleotides' (PCR03).

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  • polyacrylamide gel protocol Related Stories | Echemi

    2021-5-21 · View news and stories of polyacrylamide gel protocol. Echemi provides huge amount of polyacrylamide gel protocol information to support you.

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  • Running agarose and polyacrylamide gels

    2011-6-17 · The basics. Agarose gels can be used to resolve large fragments of DNA. Polyacrylamide gels are used to separate shorter nucleic acids, generally in the range of 1−1000 base pairs, based on the concentration used (Figure 1). These gels can be run with or without a denaturant. Gels that are run without a denaturant are referred to as native gels.

    Get Price
  • precio anionic polyacrylamide gel | Industrial water ...

    Anionic Polyacrylamide, Anionic Polyacrylamide Suppliers and Manufacturers at Alibaba.com. Alibaba.com offers 9,389 anionic polyacrylamide products. About 67% of these are paper chemicals, 65% are petroleum additives, and 50% are leather auxiliary agents. A wide variety of anionic polyacrylamide options are available to you, such as coating ...

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  • Protocols - Western Transfer (Western Blot)

    Transfer Protocol: Load the protein sample onto a 4-20% Tris-Glycine polyacrylamide gel and run until desired resolution is achieved. (The electrophoresis can be followed by using pre-stained molecular weight markers). Set up the Submerged Western Transfer Cassette as described below and in Figure III-2:

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  • Polyacrylamide - an overview | ScienceDirect Topics

    Polyacrylamide hydrogel (PAH) is an extensively cross-linked polymeric soft tissue filler substance, that has been used in the Ukraine, Russia, and China for the past 15–20 years (Christensen et al., 2003; Christensen and Breiting, 2006 ). It was originally introduced to aesthetic surgery under the name of Royamid in the Ukraine in the late ...

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  • QIAquick Gel Extraction Kit - Qiagen

    2021-2-5 · The QIAEX II Handbook contains a protocol for Polyacrylamide Gel Extraction. A specialized User-Developed Protocol (QQ05) is available when using the QIAquick Gel Extraction Kit for this purpose. Both protocols require the preparation of a diffusion buffer and a disposable plastic column or syringe barrel containing a Whatman GF/C filter or ...

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  • hydrogel medical polyacrylamide buy anionic in mexico ...

    anionicpolyacrylamide Product features anionic polyacrylamide series product is easily soluble in water and nearly insoluble in benzene, diethyl ether and acetone. It is widely used in oil extraction, mineral separation, coal washing, metallurgy, chemical, paper making, textile, sugar making, medical, construction material and agricultural ...

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