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cationic polyacrylamide vs agarose gel in netherlands

  • Agarose versus Polyacrylamide: Not All Gels Are

    1981-7-3 · Simple gel apparatus for horizontal polyacrylamide and agarose gel electrophoresis

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  • Running agarose and polyacrylamide gels

    Gels can be made with a single, continuous percentage throughout the gel (single-percentage gels), or they can be cast with a gradient of %T through the gel (gradient gels). Typical gel compositions are between 7.5 and 20% for single-percentage gels, and typical gradients are 4–15% and 10–20%.

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  • Simple gel apparatus for horizontal polyacrylamide

    Normal polyacrylamide gels (i.e., cross-linked with standard amounts of Bis, 3%–5%) have porosities that decrease linearly with the total amount of monomers (%T: total monomer concentration per 100 mL of solution) in them and which are substantially smaller than the corresponding agarose gels.

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  • Introduction to Polyacrylamide Gels | LSR | Bio-Rad

    If polyacrylamide gel is used, it is called capillary polyacrylamide gel electrophoresis (C-PAGE). In addition, other types of gels, such as Dextran, agarose gel, and poly(ethyleneoxide), can also be used. Small molecules can pass through the gel pore and are …

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  • Polyacrylamide - an overview | ScienceDirect Topics

    Polyacrylamide gel electrophoresis (PAGE) is a highly reliable and widely used technique for the separation, identification and characterization of proteins and protein mixtures. Although two-dimensional (2D)-PAGE, which combines protein isoelectric focusing (IEF) in the first dimension with sodium dodecyl sulfate (SDS)-PAGE molecular sieving in the second dimension, provides the highest resolution allowing one to separate 1000–2000 individual polypeptide spots on a single gel…

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  • Polyacrylamide Polymer - an overview | ScienceDirect

    1976-1-2 · Complex formation in vitro between human α2-macroglobulin and the human proteases cationic trypsin, chymotrypsin, plasmin and granulocyte elastase and…

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  • Polyacrylamide Gel Electrophoresis - an overview ...

    2020-7-11 · Polyacylamides are high molecular weight water soluble or swellable polymers formed from acrylamide or its derivatives. Their glass transition temperature is well above room temperature (> 400 K). The only commercially important polyacrylamide is poly(2-propenamide) which is simply called polyacrylamide or PAM [-CH 2 CH(CONH 2)-]. It is a non-ionic, watersoluble, and biocompatible …

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  • Demonstration and semiquantitative determination of ...

    2021-6-14 · The SUP gene was excised from the pCloneJET vector by restriction digestion and run on a 1% agarose gel in TAE buffer (per 1 L, 108 g Tris base, 57.1 mL …

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  • Cationic ionic liquid surfactant-polyacrylamide gel ...

    2017-9-15 · Cationic ionic liquids-based surfactants (ILS), such as 4-methyl pyridinium bromide (C n PBr, where n = 4,6,8), were used in preparation of polyacrylamide gels, sample buffer, and running buffer for cationic ILS polyacrylamide gel electrophoresis (ILS-PAGE). These ILS are liquids in the pure state and were selected for improved separation of ribonuclease b (Rib b) glycoforms in a single step ...

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  • Introduction to Polyacrylamide Gels | LSR | Bio-Rad

    2019-7-22 · After the prepared gel lane was layered on the second slab gel, a metal-free 1% agarose gel was used to fill in the space between the first and second gels to prevent diffusion of proteins.

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  • Two-Dimensional Polyacrylamide Gel Electrophoresis

    Find Cationic Polyacrylamide and related products for scientific research at MilliporeSigma

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  • Cationic Polyacrylamide | Sigma-Aldrich

    Agarose की तरह, polyacrylamide भी आणविक जीव विज्ञान में एक समान प्रक्रिया में एक महत्वपूर्ण संकल्प उपकरण के रूप में प्रयोग किया जाता है जिसे poly कहा जाता हैPolyacrylamide gel ...

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  • AGAROSE और POLYACRYLAMIDE के बीच अंतर |

    Polyacrylamide (PAA) Polyacrylamides are water-soluble synthetic linear polymers made of acrylamide or the combination of acrylamide and acrylic acid. Polyacrylamide finds applications in pulp and paper production, agriculture, food processing, mining, and as a flocculant in wastewater treatment. Polyacrylamide undergoes thermal degradation at ...

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  • Polyacrylamide - an overview | ScienceDirect Topics

    Diana L. Holmes, Nancy C. Stellwagen, Estimation of polyacrylamide gel pore size from Ferguson plots of linear DNA fragments. II. Comparison of gels with different crosslinker concentrations, added agarose and added linear polyacrylamide, ELECTROPHORESIS, 10.1002/elps.1150120903, 12, 9, (612-619), (2005).

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  • Refractive index increments of polyacrylamide and

    2021-6-14 · The SUP gene was excised from the pCloneJET vector by restriction digestion and run on a 1% agarose gel in TAE buffer (per 1 L, 108 g Tris base, 57.1 mL …

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  • Gel filtration or gel exclusion chromatography

    Electrophoresis for western blot. Electrophoresis is used to separate and analyze macromolecules based on their size and charge. Our electrophoresis protocol includes the preparation of PAGE gels and loading controls. Print this protocol. Electrophoresis can be one dimensional (i.e. …

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  • Polyacrylamide Gel Electrophoresis | Cleaver Scientific

    Agarose की तरह, polyacrylamide भी आणविक जीव विज्ञान में एक समान प्रक्रिया में एक महत्वपूर्ण संकल्प उपकरण के रूप में प्रयोग किया जाता है जिसे poly कहा जाता हैPolyacrylamide gel ...

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  • AGAROSE और POLYACRYLAMIDE के बीच अंतर |

    2019-5-25 · Estimation of polyacrylamide gel pore size from Ferguson plots of linear DNA fragments. II. Comparison of gels with different crosslinker concentrations, added agarose and added linear polyacrylamide. Electrophoresis 1991, 12 (9) , 612-619. DOI: 10.1002/elps.1150120903.

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  • Accurate molecular weight determinations of ...

    Electrophoresis for western blot. Electrophoresis is used to separate and analyze macromolecules based on their size and charge. Our electrophoresis protocol includes the preparation of PAGE gels and loading controls. Print this protocol. Electrophoresis can be one dimensional (i.e. …

    Get Price
  • Gel filtration or gel exclusion chromatography

    The gel electrophoresis market is segmented broadly into agarose gel electrophoresis and polyacrylamide gel electrophoresis. In 2019, the agarose gel electrophoresis segment dominated the market in terms of adoption. This gel system has the highest adoption in academic and research institutes which are the largest end user segment of this market.

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  • Gel electrophoresis - Tan - 2007 - Biochemistry and ...

    A discontinuous polyacrylamide and agarose gel electrophoresis system is provided which allows the fine separation of proteins based on molecular weight with the concomitant retention of native enzymatic activity. This system, referred to as the CAT gel, uses the cationic detergent CTAB, and includes a stacking gel based on a zwitterion such as ...

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  • Electrophoresis for western blot | Abcam

    2009-1-29 · Agarose and Gradient Polyacrylamide Gel Electrophoresis. Agarose gel electrophoresis of the sulfated GAG in the discontinuous buffer barium acetate/1,3-diaminopropane acetate was performed as previously described . The average molecular masses of crude and purified GAGs were analyzed by polyacrylamide linear gradient resolving gels prepared and ...

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  • Electrophoresis Market - Global Forecast to 2025 ...

    The volume of a cylindrical polyacrylamide gel was measured when immersed in a binary mixture of isobutyric acid–water at different temperatures and weight fractions of isobutyric acid. Near the upper critical solution temperature of the binary mixture, the curve for gel volume vs. isobutyric acid weight fraction has a shoulder or a peak near ...

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  • types of cationic polyacrylamide and agarose gel

    The agarose method produced banding patterns and isoelectric points which corresponded to the polyacrylamide gel data for most samples. Differences were observed for HMS-1 and PSE-1 beta-lactamases. The HMS-1 sample produced two highly resolvable enzyme bands in agarose gels rather than the single faint enzyme band observed on polyacrylamide …

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  • DNA/RNA Electrophoresis - Alfa Aesar

    2021-7-3 · DNA/RNA Electrophoresis. Gel electrophoresis separates fragments of nucleic acid that differ in size, charge or conformation. When charged molecules are placed in an electric field, they migrate toward either the positive or negative pole according to their charge. In contrast to proteins, which can have either a net positive or net negative ...

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  • Agarose gel electrophoresis of DNA | Cleaver Scientific

    2021-7-13 · Three types of gel are used in the electrophoresis process: Agarose gels have low resolution but a high range of separation. They are employed in DNA fragment analysis. Polyacrylamide gels are used for protein and small-fragment DNA analysis. Starch gels are formed from partially hydrolyzed potato starch. They are slightly more opaque than ...

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  • Gel Electrophoresis Equipment Selection Guide |

    Isolation of DNA Fragments from Polyacrylamide Gels by the Crush and Soak Method (Protocol summary only for purposes of this preview site) The standard method to recover fragments of DNA from polyacrylamide gels is the crush and soak technique originally described by Maxam and Gilbert (1977).The eluted DNA is generally free of contaminants that inhibit enzymes or that are toxic to …

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  • DNA Stains | Life Science Education | Bio-Rad

    The gel electrophoresis market is segmented broadly into agarose gel electrophoresis and polyacrylamide gel electrophoresis. In 2019, the agarose gel electrophoresis segment dominated the market in terms of adoption. This gel system has the highest adoption in academic and research institutes which are the largest end user segment of this market.

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  • Isolation of DNA Fragments from Polyacrylamide Gels

    2021-6-4 · A 1% agarose gel has pores of around 150nm, poly acrylamide (which is usually used) have 3-6nm depending on the degree of polymerisation and gel strength. You will only use agarose gels for really high molecular weight proteins otherwise you won't see much separation of the sample.

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  • Electrophoresis Market - Global Forecast to 2025 ...

    NONDISSOCIATING CATIONIC IMMUNE COMPLEXES CAN DEPOSIT IN GLOMERULAR BASEMENT MEMBRANE. Gloria Gallo. Related Papers. Antigen size influences the type of glomerular pathology in chronic serum sickness. By Pancras C . W . Hogendoorn. The pathogenesis of glomerulonephritis. By John Stewart Cameron.

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  • 1D and 2D Protein Electrophoresis: optimization and ...

    2009-12-7 · Cationic Detergent PAGEFor the analysis of very hydrophobic proteins First paper: MacFarlane D. Two dimensional benzyldimethyl-n-hexadecylammonium chloride – sodium dodecyl sulfate preparative polyacrylamide gel electrophoresis: a high capacity high resolution technique for the purification of proteins from complex mixtures.

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  • Vesicle formation between single-chained cationic ...

    2014-8-9 · Vesicle formation in plasmid DNA/single-chained cationic surfactant system. The turbidities for single-chained surfactant solution [26, 27] and plasmid DNA solution (indicated by the optical density at 514 nm, shown in curve a, Fig. 2a) are negligible.However, plasmid DNA/surfactant system exhibits obvious turbidity (Fig. 2a, curve b and Figs. 2b–c and S1) after plasmid DNA concentration ...

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  • Electrophoresis Market - Global Forecast to 2025 ...

    The gel electrophoresis market is segmented broadly into agarose gel electrophoresis and polyacrylamide gel electrophoresis. In 2019, the agarose gel electrophoresis segment dominated the market in terms of adoption. This gel system has the highest adoption in academic and research institutes which are the largest end user segment of this market.

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  • DNA & RNA Precipitation Solutions - Gene Link

    2018-6-4 · nuclease by using these in RNA and DNA precipitation followed by agarose gel electrophoresis to determine integrity and no breakdown of nucleic acids. Appropriate molarity solutions of LiCl, sodium acetate, potassium acetate and sodium chloride are prepared in nuclease free autoclaved water and autoclaved again after preparation.

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  • Protein Electrophoresis Methods | LSR | Bio-Rad

    2009-1-29 · Agarose and Gradient Polyacrylamide Gel Electrophoresis. Agarose gel electrophoresis of the sulfated GAG in the discontinuous buffer barium acetate/1,3-diaminopropane acetate was performed as previously described . The average molecular masses of crude and purified GAGs were analyzed by polyacrylamide linear gradient resolving gels prepared and ...

    Get Price
  • Isolation and Characterization of Low Molecular

    Isolation of DNA Fragments from Polyacrylamide Gels by the Crush and Soak Method (Protocol summary only for purposes of this preview site) The standard method to recover fragments of DNA from polyacrylamide gels is the crush and soak technique originally described by Maxam and Gilbert (1977).The eluted DNA is generally free of contaminants that inhibit enzymes or that are toxic to …

    Get Price
  • Isolation of DNA Fragments from Polyacrylamide Gels

    Bis-Tris gel chemistry . The conditions for electrophoresis (pH and buffers) are more favorable with Bis-Tris chemistry-based gels. These gels are HCI buffered and have a neutral operating pH. The running buffer can either be MES (50mM, with 50mM Tris) at pH 7.2 or MOPS (with Tris) at pH 7.7. Compared to Tris-Glycine gels, these gels employ ...

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  • Tris-Glycine vs Bis-Tris Gel Chemistry | Abcam

    -Stacking gel is on top of running gel.-Lower concentration of acrylamide (large pore size) (4%).-Lower pH (~6.8).-Different ionic content.(chlorine ions)-Concentrates the proteins in a lane into a tight band before they enter the running gel.-Results in better separated …

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  • Lab 5 SDS-PAGE(Sodium-Dodecyl-Sulfate

    2021-7-15 · This article throws light upon the top ten types of electrophoretic techniques used in biochemistry. The ten types of electrophoretic techniques used in biochemistry are: (1) Horizontal and Vertical Gel Electrophoresis Systems (2) Agarose Gel Electrophoresis (3) Polyacrylamide Gels (4) Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (5) Native (Buffer) Gels (6) Gradient …

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  • Complexation to Cationic Microspheres of Double

    2021-3-5 · lyzed by agarose gel electrophoresis. The resistance of 32p-labeled DNA-DNA and DNA-PDP molecules in the presence of serum or cellular extracts was evaluated after binding to CM by gel electrophoresis analysis. DDAB18 Eudragit RS 100 microspheres are able to bind to

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  • DNA/RNA Electrophoresis - Alfa Aesar

    2021-7-3 · DNA/RNA Electrophoresis. Gel electrophoresis separates fragments of nucleic acid that differ in size, charge or conformation. When charged molecules are placed in an electric field, they migrate toward either the positive or negative pole according to their charge. In contrast to proteins, which can have either a net positive or net negative ...

    Get Price
  • DNA & RNA Precipitation Solutions - Gene Link

    2018-6-4 · nuclease by using these in RNA and DNA precipitation followed by agarose gel electrophoresis to determine integrity and no breakdown of nucleic acids. Appropriate molarity solutions of LiCl, sodium acetate, potassium acetate and sodium chloride are prepared in nuclease free autoclaved water and autoclaved again after preparation.

    Get Price
  • 1D and 2D Protein Electrophoresis: optimization and ...

    2009-12-7 · Cationic Detergent PAGEFor the analysis of very hydrophobic proteins First paper: MacFarlane D. Two dimensional benzyldimethyl-n-hexadecylammonium chloride – sodium dodecyl sulfate preparative polyacrylamide gel electrophoresis: a high capacity high resolution technique for the purification of proteins from complex mixtures.

    Get Price
  • A Novel, Modification-Dependent ATP-Binding Aptamer ...

    2020-7-17 · ATP. The unreacted epoxy-agarose was quenched by reacting it with mercaptoethanol (10 mM, pH 8.0) for 16 h. The ATP-agarose was washed with 5 column volumes of 1 M NaCl and stored in 20 mM Tris-HCl (pH 7.12) and 1 M NaCl as a 50% slurry at 4 °C. A preselection agarose gel was prepared from epoxy-agarose by treatment with mercaptoethanol (10

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  • Tris-Glycine vs Bis-Tris Gel Chemistry | Abcam

    Bis-Tris gel chemistry . The conditions for electrophoresis (pH and buffers) are more favorable with Bis-Tris chemistry-based gels. These gels are HCI buffered and have a neutral operating pH. The running buffer can either be MES (50mM, with 50mM Tris) at pH 7.2 or MOPS (with Tris) at pH 7.7. Compared to Tris-Glycine gels, these gels employ ...

    Get Price
  • Gel Electrophoresis Equipment Selection Guide |

    2021-7-13 · Three types of gel are used in the electrophoresis process: Agarose gels have low resolution but a high range of separation. They are employed in DNA fragment analysis. Polyacrylamide gels are used for protein and small-fragment DNA analysis. Starch gels are formed from partially hydrolyzed potato starch. They are slightly more opaque than ...

    Get Price
  • Gels | September 2020 - Browse Articles - MDPI

    The volume of a cylindrical polyacrylamide gel was measured when immersed in a binary mixture of isobutyric acid–water at different temperatures and weight fractions of isobutyric acid. Near the upper critical solution temperature of the binary mixture, the curve for gel volume vs. isobutyric acid weight fraction has a shoulder or a peak near ...

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  • Denaturing Gradient Gel Electrophoresis | Cleaver

    The design of the mRNA vaccine involves the selection of in vitro transcription (IVT) systems and nonviral delivery vectors. This study aimed to verify the effect of 5’ and 3’ untranslated region (UTR) sequences on the translation efficiency of mRNA. Three modes of IVT-mRNA systems (IVT-mRNA-n1/n2/n3) with diverse UTRs were constructed, and EGFP (enhanced green fluorescent protein) and HA ...

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  • 1D and 2D Protein Electrophoresis: optimization and ...

    2009-12-7 · Cationic Detergent PAGEFor the analysis of very hydrophobic proteins First paper: MacFarlane D. Two dimensional benzyldimethyl-n-hexadecylammonium chloride – sodium dodecyl sulfate preparative polyacrylamide gel electrophoresis: a high capacity high resolution technique for the purification of proteins from complex mixtures.

    Get Price
  • Electrophoresis | Lonza

    2013-6-21 · Complex formation between Nps and plasmid DNA was analyzed by 1% agarose gel electrophoresis. Free plasmid DNA (4 μg) and DNA peptide Nps complexes with peptide/DNA ratio of 20/1 (4 μg DNA/80 μg of peptide in 40 μl nuclease free water) were run on a 1% (w/v) agarose gel with TBE buffer (89 mmol/l Tris, 89 mmol/l boric acid, and 2 mmol/l ...

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  • Self-assembled nanoparticles based on modified

    2010-2-15 · in a gel with pH-gradient the particles of peptides or proteins migrate to get to the place of its isoelectric point very high resolving power: the substances differing in pI by only 0.002 can be resolved SDS-PAGE electrophoresis in polyacrylamide gel in the presence of sodium dodecylsulphate (SDS)

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  • AES Electrophoresis Society: One-Dimensional ...

    2002-5-1 · DNA mobility anomalies are determined primarily by polyacrylamide gel concentration, not gel pore size. Electrophoresis 1997, 18 (1) , 34-44. DOI: 10.1002/elps.1150180108. Mitsuhiro Shibayama, Masao Uesaka, Yasuhiro Shiwa.

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  • Analysis of peptides and proteins - vscht.cz

    1991-12-1 · Gel-Like Structure of a Hexadecyl Derivative of Hyaluronic Acid for the Treatment of Osteoarthritis. Macromolecular Bioscience 2009, 9 (7) , 646-653. DOI: 10.1002/mabi.200900009. Nancy C. Stellwagen. Electrophoresis of DNA in agarose gels, polyacrylamide gels and in free solution.

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  • Scattering and swelling properties of

    For polyacrylamide and agarose gel electrophoresis. V4251. TAE Buffer, Molecular Biology Grade. Buffer for agarose DNA electrophoresis. V4271, V4281. Ethidium Bromide Solution, Molecular Grade. Fluorescent dye suitable for staining nucleic acids after electrophoresis or in cesium chloride gradients. H5041. Blue/Orange Loading Dye, 6X

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  • Dynamic and static light scattering by aqueous ...

    A theory of the electrophoresis of DNA through gels with large interfiber spacing, such as dilute agarose, is presented. We assume that the DNA molecule moves along its axis through a “tube” in a neutral gel under the influence of the electric field. The tube is random except for possible bias due to …

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  • Agarose, Low Melting Point, Analytical Grade

    performed in 4% polyacrylamide (Biorad, California, USA) rod gels containing 2% ampholines, pH 3.5-10 (LKB, Bromma, Sweden). For the second dimension, the rod gels were applied directly onto the stacking gel of SDS-polyacrylamide gels and covered with a 1% agarose solution in running buffer.

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