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polyacrylamide gradient gel electrophoresis supplier

  • Polyacrylamide Gradient Gel | Bio-Rad | Bioz

    Polyacrylamide Gradient Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 17 PubMed citations. ZERO BIAS - scores, article …

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  • Polyacrylamide Gels | Bio-Rad | Bioz

    Bio-gel P2 resin, Econo-Pac 10 DG desalting columns, and all reagents for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were acquired from Bio-Rad Laboratories (Hercules, CA). .. Amicon YM-10 filtration products were purchased from Millipore (Billerica, MA).

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  • Protein Gels | Thermo Fisher Scientific - US

    Gradient SDS Polyacrylamide Gel Electrophoresis. The preparation of fixed-concentration polyacrylamide gels has been described in Chapter 6. However, the use of polyacrylamide gels that have a gradient of increasing acrylamide concentration (and hence decreasing pore size) can sometimes have advantages over fixed-concentration acrylamide gels.

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  • Gradient SDS Polyacrylamide Gel Electrophoresis

    1996-12-20 · A means for casting step-gradients in linear polyacrylamide gel concentration for capillary electrophoresis is presented. A UV-Vis whole-column detector is used to profile the gel gradients cast in the capillary, while detection of fluorescein-labeled proteins is accomplished with an epi-illumination laser-induced fluorescence whole-column detection system.

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  • Preparation of linear polyacrylamide gel step gradients ...

    Polyacrylamide Gel-electrophoresis across a Molecular Sieve Gradient. J. MARGOLIS 1 & K. G. KENRICK 1 Nature volume 214, pages 1334–1336 (1967)Cite this article

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  • Polyacrylamide Gel-electrophoresis across a Molecular ...

    2010-9-27 · dimensional electrophoresis to determine the molecular weight of unknown proteins (Bio-Rad Bulletin 3133). In polyacrylamide gel electrophoresis (PAGE), proteins are driven through the gel under an electric field, during which the gel matrix acts as a sieve to separate proteins based on size (SDS-PAGE), or charge and shape (native PAGE). The

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  • bts Biotech Trade & Service GmbH | GeBaGel Gradient Gels ...

    The GeBAGels combined with the GeBARunner Electrophoresis Unit is a revolutionary pre-cast polyacrylamide mini-gel system for horizontal protein electrophoresis or DNA/RNA electrophoresis in polyacrylamide gels. The GeBAGel & GeBARunner electrophoresis system are compatible with robotic loading and can be used to automate the process.

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  • Gradient Gels for SDS-PAGE | Biocompare

    Gradient gels for SDS-PAGE can be purchased from various suppliers for protein separation. Gradient gels for denaturing protein electrophoresis feature a gradually increasing polyacrylamide layer from the top to the bottom of the gel. This can allow greater resolution when separating a wide range of protein molecular weights.

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  • Polyacrylamide at Thomas Scientific

    Suitable for electrophoresis and tested as a catalyst for polyacrylamide gel electrophoresis. Related Products: Temed. Compare this item ... Used in sample preparation and gel formation for polyacrylamide gel electrophoresis. Also used in casting gradient gels, as a protein stabilizer and storage buffer component. ... By Supplier; By Category A ...

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  • 4-15% Gradient Gels | Biocompare

    4-15% Gradient Gels. Ready-to-use 4-15% gradient gels are available from several suppliers for electrophoresis applications. Gradient gels for denaturing protein electrophoresis feature a gradually increasing polyacrylamide layer from the top (4%) to the bottom (15%) of the gel. A decreasing pore size can allow greater resolution when ...

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  • Polyacrylamide temperature gradient gel electrophoresis ...

    Key words Temperature gradient gel electrophoresis, DNA, RNA, Protein, Polyacrylamide, Gel staining, Dye 1 Introduction Temperature Gradient Gel Electrophoresis (TGGE) was first described by Roger Wartell from Georgia Institute of Technology, and extensive initial work was carried out by the Riesner laboratory in Germany [1].

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  • Polyacrylamide Gel Electrophoresis | Cleaver Scientific

    2003-12-1 · Gradient gel electrophoresis for LDL separation is not a simple method and high-quality non-denaturing gradient gels are lacking. Methods: In this paper, we describe a method for the preparation of a polyacrylamide gel system that consists of an upper linear gradient gel (1.8–10%) and a lower homogeneous gel (16%) for the determination of LDL ...

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  • CytivaSG Series Gradient Makers:Gel Electrophoresis ...

    1996-12-20 · A means for casting step-gradients in linear polyacrylamide gel concentration for capillary electrophoresis is presented. A UV-Vis whole-column detector is used to profile the gel gradients cast in the capillary, while detection of fluorescein-labeled proteins is accomplished with an epi-illumination laser-induced fluorescence whole-column detection system.

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  • Gradient Gels for SDS-PAGE | Biocompare

    Gradient gels for SDS-PAGE can be purchased from various suppliers for protein separation. Gradient gels for denaturing protein electrophoresis feature a gradually increasing polyacrylamide layer from the top to the bottom of the gel. This can allow greater resolution when separating a wide range of protein molecular weights.

    Get Price
  • VWR Life Science NEXT GEL® Polyacrylamide Gel ...

    VWR Life Science NEXT GEL® Polyacrylamide Gel Electrophoresis Solutions. NEXT GEL® is a ready to pour pre-mixed solution of acrylamide, bisacrylamide, gel buffer, and SDS that enables superior, ultra-fine resolution of protein bands. The unique acrylamide matrix slows the migration of proteins, eliminating the need for a stacking gel.

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  • Polyacrylamide Gel Electrophoresis | Cleaver Scientific

    Polyacrylamide gradient gel electrophoresis appears suitable to detect and quantitatively evaluate cytosolic retinol‐ and retinoic acid‐binding proteins. Distribution and cellular levels of retinol‐binding protein and retinoic acid‐binding protein, involved in the molecular action of retinoids, were analyzed in rat testis and liver.

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  • Polyacrylamide gradient gel electrophoresis of cytosolic ...

    Gel opening lever ( 456-0000 ), sold separately, is 100% aluminum and recyclable. Ready Gel polyacrylamide precast gels are designed to fit the Mini-PROTEAN ® Tetra cell and are ready to run. Simply lock them into the cell, load your samples, and get sharp, beautifully resolved protein bands in …

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  • UltraPure™ Acrylamide - Thermo Fisher Scientific

    Polyacrylamide gel electrophoresis (PAGE) Polyacrylamide gel electrophoresis (PAGE) is a foundational technique used to separate proteins and other macromolecules by their electrophoretic mobility. Polyacrylamide gels are formed through the polymerization of acrylamide monomers and bis-acrylamide crosslinking molecules.

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  • Polyacrylamide Reagents and Precast Gels | 생명 과학 교육 ...

    2020-5-29 · gradient could be established throughout the gel and the frictional retarding force of the gel rendered insignificant, then the molecules could be separated on the basis of charge alone . This is the basic principle of Polyacrylamide Gel Electrophoresis (PAGE) and its various applications using gradient and 2D gels .

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  • Hand-casting gels for PAGE and SDS-PAGE using TurboMix

    NuPAGE Tris-Acetate protein gels come in a polyacrylamide concentration of 7% and a 3–8% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Tris-Acetate gels also come in multiple well formats.

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  • bts Biotech Trade & Service GmbH | GeBaGel Gradient Gels ...

    The GeBAGels combined with the GeBARunner Electrophoresis Unit is a revolutionary pre-cast polyacrylamide mini-gel system for horizontal protein electrophoresis or DNA/RNA electrophoresis in polyacrylamide gels. The GeBAGel & GeBARunner electrophoresis system are compatible with robotic loading and can be used to automate the process.

    Get Price
  • GRADIENT GELS FOR NATIVE-PAGE - The Slab Gel

    4-15% Gradient Gels. Ready-to-use 4-15% gradient gels are available from several suppliers for electrophoresis applications. Gradient gels for denaturing protein electrophoresis feature a gradually increasing polyacrylamide layer from the top (4%) to the bottom (15%) of the gel. A decreasing pore size can allow greater resolution when ...

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  • 4-15% Gradient Gels | Biocompare

    Abstract A comparison of the distribution patterns of glycoproteins released into the medium by normal and oncogenic virus-transformed mammalian cells on 5% and 2–12% linear gradient sodium dodecylsulfate-polyacrylamide gels was made. Marked differences were observed in the distribution patterns obtained with the two types of gels. The gradient gels separate the …

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  • Polyacrylamide Gel Electrophoresis | Cleaver Scientific

    2020-5-29 · gradient could be established throughout the gel and the frictional retarding force of the gel rendered insignificant, then the molecules could be separated on the basis of charge alone . This is the basic principle of Polyacrylamide Gel Electrophoresis (PAGE) and its various applications using gradient and 2D gels .

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  • Polyacrylamide gel electrophoresis of glycoproteins on ...

    NuPAGE Tris-Acetate protein gels come in a polyacrylamide concentration of 7% and a 3–8% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Tris-Acetate gels also come in multiple well formats.

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  • Electrophoresis - MP Bio

    2009-9-7 · Molecular formula : MW : CAS : nature : as polyacrylamide gel electrophoresis support of the media. Gel through the acrylamide monomer and crosslinking agent-methylene - bis - acrylamide in the catalyst and accelerator under polymerization, it has good repeatability, and distinguish between strong and gel adjustable aperture size and chemical properties of stability, …

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  • NuPAGE™ 3 to 8%, Tris-Acetate, 1.0 mm, Mini Protein Gel ...

    An improved method for the estimation of molecular weights of native proteins by polyacrylamide gel electrophoresis, in 9 cm × 9 cm × 0.05 mm 4–20% T fabric reinforced gradient gels, is described. Plotting the logarithm of the relative mobilities of proteins versus gel concentrations produces lines whose slopes are related to molecular weights.

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  • polyacrylamide gel electrophoresis;PAGE,physical ...

    Several methods, such as sequential ultracentrifugation, chemical precipitation, immunoaffinity chromatography, and nondenaturing polyacrylamide gradient gel electrophoresis, have been used to separate HDL subfractions (8).

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  • GRADIENT GELS FOR SDS-PAGE - Slab Gel Electrophoresis

    So, each gradient gel has a zone or layer of specific gel strength. These gradient gels are prepared under the supervision of the qualified scientists. They are cost effective and highly reproducible with shelf life of 3-4 months at 4ºC. The gel cassettes fit with commercially available vertical gel electrophoresis system such as: Hoefer-GE ...

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  • anionic polyacrylamide gradient gel electrophoresis ...

    Polyacrylamide gradient gel electrophoresis appears suitable to detect and quantitatively evaluate cytosolic retinol‐ and retinoic acid‐binding proteins. Distribution and cellular levels of retinol‐binding protein and retinoic acid‐binding protein, involved in the molecular action of retinoids, were analyzed in rat testis and liver.

    Get Price
  • bts Biotech Trade & Service GmbH | GeBaGel Gradient Gels ...

    The GeBAGels combined with the GeBARunner Electrophoresis Unit is a revolutionary pre-cast polyacrylamide mini-gel system for horizontal protein electrophoresis or DNA/RNA electrophoresis in polyacrylamide gels. The GeBAGel & GeBARunner electrophoresis system are compatible with robotic loading and can be used to automate the process.

    Get Price
  • Electrophoresis - MP Bio

    2020-5-29 · gradient could be established throughout the gel and the frictional retarding force of the gel rendered insignificant, then the molecules could be separated on the basis of charge alone . This is the basic principle of Polyacrylamide Gel Electrophoresis (PAGE) and its various applications using gradient and 2D gels .

    Get Price
  • Polyacrylamide gel electrophoresis of glycoproteins on ...

    Abstract A comparison of the distribution patterns of glycoproteins released into the medium by normal and oncogenic virus-transformed mammalian cells on 5% and 2–12% linear gradient sodium dodecylsulfate-polyacrylamide gels was made. Marked differences were observed in the distribution patterns obtained with the two types of gels. The gradient gels separate the …

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  • Agarose and polyacrylamide gel electrophoresis methods

    2011-5-27 · 05: Supplemental Figure 4 Extended electrophoresis of HA standards on a 4–20% gradient polyacrylamide gel in TBE buffer.The lowest molecular mass HA fragments have been electrophoresed off the gel bottom. HA samples were LoLadder™ (495, 310, 214, 110, 30 kDa HA) at loads of 0.5 (left lane) and 1.0 µg (right lane).

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  • Determination of molecular weight of native proteins by ...

    An improved method for the estimation of molecular weights of native proteins by polyacrylamide gel electrophoresis, in 9 cm × 9 cm × 0.05 mm 4–20% T fabric reinforced gradient gels, is described. Plotting the logarithm of the relative mobilities of proteins versus gel concentrations produces lines whose slopes are related to molecular weights.

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  • BASIC PROTOCOL: PURIFICATION OF

    2016-8-9 · The speed of electrophoresisis directly proportional to the voltage gradient across the gel. The currentin the circuit and the heat generated for higher percentage gels (>15 %acrylamide) are corresponding smaller since the increased acrylamide concentrationleads to greater resistance. ... by polyacrylamide gel electrophoresis. Biochemistry14 ...

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  • Separation of RNA according to Size: Electrophoresis of ...

    In contrast to electrophoresis using agarose gels, which occurs while the gel is horizontal, polyacrylamide gels are run while in the vertical position. Gels are also typically run at 45°C–55°C, which is the melting temperature of RNA, and in the presence of 6–8 m urea.

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  • Determination of molecular weight of native proteins by ...

    An improved method for the estimation of molecular weights of native proteins by polyacrylamide gel electrophoresis, in 9 cm × 9 cm × 0.05 mm 4–20% T fabric reinforced gradient gels, is described. Plotting the logarithm of the relative mobilities of proteins versus gel concentrations produces lines whose slopes are related to molecular weights.

    Get Price
  • bts Biotech Trade & Service GmbH | GeBaGel Gradient Gels ...

    The GeBAGels combined with the GeBARunner Electrophoresis Unit is a revolutionary pre-cast polyacrylamide mini-gel system for horizontal protein electrophoresis or DNA/RNA electrophoresis in polyacrylamide gels. The GeBAGel & GeBARunner electrophoresis system are compatible with robotic loading and can be used to automate the process.

    Get Price
  • anionic polyacrylamide gradient gel electrophoresis ...

    Polyacrylamide gradient gel electrophoresis appears suitable to detect and quantitatively evaluate cytosolic retinol‐ and retinoic acid‐binding proteins. Distribution and cellular levels of retinol‐binding protein and retinoic acid‐binding protein, involved in the molecular action of retinoids, were analyzed in rat testis and liver.

    Get Price
  • Polyacrylamide temperature gradient gel electrophoresis ...

    Key words Temperature gradient gel electrophoresis, DNA, RNA, Protein, Polyacrylamide, Gel staining, Dye 1 Introduction Temperature Gradient Gel Electrophoresis (TGGE) was first described by Roger Wartell from Georgia Institute of Technology, and extensive initial work was carried out by the Riesner laboratory in Germany [1].

    Get Price
  • Denaturing Gradient Gel Electrophoresis - Topac

    2017-2-2 · Gradient blue native polyacrylamide gel electrophoresis (BN-PAGE) is a well established and widely used technique for activity analysis of high-molecular-weight proteins, protein complexes, and protein-protein interactions. Since its inception in the early 1990s, a variety of minor modifications have been made to this gradient gel analytical ...

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  • Non‐Gradient Blue Native Polyacrylamide Gel Electrophoresis

    SDS-polyacrylamide gel electrophoresis 2 4 Electrophoresis 12 5 Preparations 3 6. Comparison 3 7 immunoblot analysis 2 8 preserved 3 9 starch-gel electrophoresis 20 10 activated 2 11 polyacrylamide 3 12 gels 12 13 proteins 4 14 gel electrophoresis 38 15 tube ...

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  • Two-dimensional Resolution of Plasma Proteins by ...

    Two-dimensional Resolution of Plasma Proteins by Combination of Polyacrylamide Disc and Gradient Gel Electrophoresis

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  • Electrophorèse - um c

    2020-3-18 · gradient de pH) la migration dépend uniquement du pI -dans l'électrophorèse 'SDS -PAGE'*, la vitesse de migration . dépend - de la taille de sous-unité (protéine dénaturée) - du degré de réticulation du gel * Sodium dodecyl sulfate polyacrylamide gel . electrophoresis . 9

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  • Nondenaturing polyacrylamide gradient gel electrophoresis

    Nondenaturing polyacrylamide gradient gel electrophoresis NICHOLS AV Methods Enzymol 128, 417-433, 1986

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  • CERTIFICATE OF ANALYSIS

    2017-12-27 · Polyacrylamide Gradient Gel Electrophoresis. To show one band only at approximately 100kD corresponding to PAP molecular weight. Pass/Fail. Cellulose Acetate Electrophoresis. To show one band with β 1 electrophoretic mobility. Pass/Fail. Bioburden. < 100 CFU/ml. Pass/Fail. Physical appearance White, lyophilised powder.

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  • anionic polyacrylamide gradient gel electrophoresis ...

    Polyacrylamide gradient gel electrophoresis appears suitable to detect and quantitatively evaluate cytosolic retinol‐ and retinoic acid‐binding proteins. Distribution and cellular levels of retinol‐binding protein and retinoic acid‐binding protein, involved in the molecular action of retinoids, were analyzed in rat testis and liver.

    Get Price
  • VWR Life Science NEXT GEL® Polyacrylamide Gel ...

    VWR Life Science NEXT GEL® Polyacrylamide Gel Electrophoresis Solutions. NEXT GEL® is a ready to pour pre-mixed solution of acrylamide, bisacrylamide, gel buffer, and SDS that enables superior, ultra-fine resolution of protein bands. The unique acrylamide matrix slows the migration of proteins, eliminating the need for a stacking gel.

    Get Price
  • Gradient SDS Polyacrylamide Gel Electrophoresis of ...

    1994-1-1 · The preparation of fixed-concentration polyacrylamide gels has been described in Chapter 5 . However, the use of polyacrylamide gels that have a gradient of increasing acrylamide concentration (and hence decreasing pore size) can sometimes have advantages over fixed-concentration acrylamide gels. During electrophoresis in gradient gels, proteins migrate until the …

    Get Price
  • Study of LDL by polyacrylamide gradient gel electrophoresis

    2009-3-31 · Study of LDL by polyacrylamide gradient gel electrophoresis. 1) Testing Department A Laboratory, Mitsubishi Kagaku Bioclinical Laboratories, Inc. 2) [in Japanese] We examinied the differentiation of lipoprotein in human serum using polyacrylamide gradient gel (PAGG) electrophoresis to analyze the lipid components and to quantify the small ...

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  • HIGH-RESOLUTION GRADIENT POLYACRYLAMIDE

    2011-1-5 · ABSTRACT. A procedure for high resolution electrophoresis of isozymes of the mosquito Aedes aegypti using nonlinear (2.570-20%) polyacrylamide gradient gel slabs is described. Crude mosquito homogenates were electrophoresed on gradient and on homogeneous (7%) polyacrylamide gels and stained for esterase (EST),

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  • The principle and method of polyacrylamide gel ...

    A method for measuring lymph-to-plasma (L/P) protein concentration ratios obtained from protein fractions separated by polyacrylamide gradient gel electrophoresis is presented. A curve-fitting technique is used to decompose lymph and plasma electropherograms containing multiple components into individual components, eliminating protein-protein ...

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  • Computer analysis of proteins separated by polyacrylamide ...

    2017-2-2 · Gradient blue native polyacrylamide gel electrophoresis (BN-PAGE) is a well established and widely used technique for activity analysis of high-molecular-weight proteins, protein complexes, and protein-protein interactions. Since its inception in the early 1990s, a variety of minor modifications have been made to this gradient gel analytical ...

    Get Price
  • Non‐Gradient Blue Native Polyacrylamide Gel Electrophoresis

    2016-8-9 · Purificationof DNA using nondenaturing polyacrylamide gel electrophoresis. JackD. Pollard, Jr. 4/12/98. ... To separate fragmentsover a wide range of molecular weights, a pore-gradient gel can be used.In such a gel the pore size is larger at the top than at the bottom, andthe gel becomes more restrictive as the fragment runs down the gel. ...

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  • Purificationof DNA using nondenaturing polyacrylamide gel ...

    simplified method of denaturing gradient gel electrophoresis (18) that had been developed by Myers et al. (19). As analysis of mobility shift [probably due to a conformational change of single-stranded DNAs on polyacrylamide gel electrophoresis (20)] has been used to detect point mutations (21), in this work

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