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polyacrylamide quantification in cuba

  • Novel and sensitive ELISA for the rapid quantification

    The reference curve showed to be precise and accurate over the entire linear range of 1.25 and 20ng/mL with a limit of quantification validated to 1.25ng/mL. The intra- and inter-assay coefficient of variation ranged from 0.35 to 6.65% and 4.70 to 10.63%, respectively.

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  • Two decades of proteomics in Latin America: A

    2014-7-31 · In small countries like Cuba, it is difficult to have advanced and very expensive instruments. However, the Cuban government strongly supported development of biotechnology and in 1987, one of the most powerful mass spectrometer (JEOL JMS-HX110) at the time was installed at the Center for Genetic Engineering and Biotechnology (CIGB), jointly with HPLC systems ( Fig. 2 ).

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  • Polyacrylamide Gel Electrophoresis | Cleaver Scientific

    Author information: (1)Process Control Department, Center for Genetic Engineering and Biotechnology, P.O. Box 6162, Havana 10600, Cuba. An enzyme-linked immunosorbent assay to quantify the mAb CB.Hep-1 during downstream purification process was standardized and validated.

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  • A rapid and sensitive ELISA to quantify an HBsAg

    At the start of the past decade a group of scientists from the Center for Genetic Engineering and Biotechnology in Havana, Cuba, described a new method that uses zinc (II) and imidazole salts for the detection of proteins separated by electrophoresis in polyacrylamide gels.

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  • [PDF] Precipitación selectiva de imidazolato de zinc ...

    2016-12-15 · Methods: Disc polyacrylamide gel electrophoresis and quantification of CSF total protein in CSF and serum of 61 patients were conducted at the Institute of Neurology and Neurosurgery (INN) between April and September 2014. The concentration of albumin and IgG was

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  • Cuantificación de la inmunoglobulina G y la albúmina en

    2021-5-31 · An electrophoretic separation of proteins (sodium dodecyl sulfate–polyacrylamide gel electrophoresis) was used to demonstrate differences in protein migration pattern in OMVs obtained from N. meningitidis B:4:P1.9 (vaccine strain), B:4:P1.15 (heterologous strain), and N. …

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  • Immunogenicity of antigens from outer membrane

    Biologics Analysis Workflow. The Biologics Analysis Workflow is a four-step solution designed and validated to assess the purity or identity of biological products in a cGMP regulatory environment. The integrated system enables expedited electrophoresis, simplified staining, easy analysis, …

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  • Fundamental Laboratory Approaches for Biochemistry

    2021-7-2 · The determination of Human Chorionic Gonadotropin (HCG) in biological fluids is of great interest in the early pregnancy diagnostics, the evaluation o…

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  • Increased levansucrase production by a genetically ...

    2012-2-10 · ABSTRACT INTRODUCTION Differentiating between HIV-1 and HIV-2 infection is the first step to understanding HIV transmission, epidemiology and pathogenesis in geographical areas where both viruses circulate. In Cuba, positive results in mixed HIV-1/2 screening assays are confirmed by HIV-1 Western blot. Indeterminate results constitute the main limitation of this test and HIV-2 infection is ...

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  • MEDICC Review | HIV‑2 Antibody Detection after ...

    Quantification of signal in a designated cellular area or volume. Open the image file with ImageJ software. Draw an area to denote the area of interest using the geometrical form function (tool bar 'rectangle,' 'oval,' or 'polygon'). NOTE: The quantification value will …

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  • Quantitative Immunofluorescence to Measure Global ...

    The intra- and interassay coefficient of variation ranged from 0.25 to 8.64% and 1.84 to 9.43%, respectively. Significant differences were not observed in the plant-derived antibody quantification by HRP-ELISA and PhoA-ELISA (n=18), demonstrating that plant endogenous peroxidases do not produce interferences in the quantification of this molecule.

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  • A rapid and sensitive ELISA to quantify an HBsAg

    Ferreras, M., Gavilanes, J. G., and Garcia-Segura, J. M. (1993) A permanent Zn 2+ reverse staining method for the detection and quantification of proteins in polyacry-lamide gels. Analyt. Biochem. 213, 206–12. PubMed CrossRef Google Scholar

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  • Zn2+-Reverse Staining Technique | SpringerLink

    2019-10-21 · Stevia rebaudiana (Asteraceae), native from Paraguay, accumulates steviol glycosides (SGs) into its leaves. These compounds exhibit acaloric intense sweet taste which answers to consumer demands for reducing daily sugar intake. Despite the developpement of S. rebaudiana cultivation all over the world, the development of new cultivars is very recent, in particular due to a colossal lack of (1 ...

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  • Genetic diversity and population structure of the

    2021-2-18 · MS-based quantification of protein biomarkers is mainly performed by a bottom-up approach using peptide fragments obtained by enzymatic protein digestion with trypsin. Standard digestion protocols require a reaction time of more than 20 hours, which is a rate-limiting factor in sample preparation workflows.

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  • Novel gel electrophoresis technique allows rapid

    2019-5-26 · The practice of quantifying proteins by peptide fragments from enzymatic proteolysis (digestion) was assessed regarding accuracy, reliability, and uncertainty of the results attainable. Purified recombinant growth hormone (rhGH, 22 kDa isoform) was used as a model analyte. Two tryptic peptides from hGH, T6 and T12, were chosen to determine the amount of the protein in the original sample.

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  • Protein Quantification by Isotope Dilution Mass ...

    2021-3-12 · Some chitinases are used for quantification of fungi in the soil, isolation of fungal protoplast, production of single-cell proteins, localization of chitin in cells, and as targets for bioinsecticides (Dahiya et al. 2005; Patil and Jadhav 2015). They play a vital role in the morphogenesis of both insects and fungi (Dahiya et al. 2005).

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  • Increased levansucrase production by a genetically ...

    2018-1-19 · Zapata y G, Havana 10400, Cuba bFinlay Institute of Vaccines, Ave 27 Nr. 19805, Havana 10600, Cuba ... and 7 % of polyacrylamide for TT, and resolving gel with 4 % of polyacrylamide. S7 ... bProtein quantification using Lowry´s method4

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  • Development of Antibacterial Glycoconjugate Vaccine ...

    2016-12-15 · Instituto de Neurología y Neurocirugía La Habana, Cuba 2Licenciada en Farmacia. Master en Neurociencias del Comportamiento. Investigador Auxiliar. Instituto de Neurología y Neurocirugía. La Habana, Cuba ... Disc polyacrylamide gel electrophoresis and quantification of CSF total protein in CSF and serum of 61 patients were conducted

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  • Cuantificación de la inmunoglobulina G y la albúmina en

    2004-5-28 · In Cuba, ER was first given in 1972 by means of biochemical methods such as dextran-coated charcoal followed by Scatchard plotting 11,12, the isoelectric approach method 13,14,15 and the sucrose-density gradient.

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  • MEDICC Review - Revention and treatment of

    Increasing investment in R&D activities and increasing number of protein quantification research projects are the factors which are responsible for the growth of this market. Sodium dodecyl sulfate polyacrylamide gel electrophoresis is a derivative of polyacrylamide gel electrophoresis, a biochemical analytical method for separating charged ...

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  • Sodium Dodecyl Sulphate-Polyacrylamide Gel

    2021-6-2 · Protein Quantification of OMVs. A total of 10 11 OMVs were diluted in 4× Laemmli buffer (Bio-Rad Laboratories Inc.) with β-mercaptoethanol (Sigma-Aldrich), denaturated at 95°C for 5 min and loaded onto 7.5% sodium dodecyl sulfate–polyacrylamide gels (SDS-PAGE). After electrophoresis, gels were stained with 25 mL of BlueSafe (NZYTech ...

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  • Frontiers | Isolation Method and Characterization of

    2016-2-11 · Techniques based on high-resolution liquid chromatography are currently widely used to quantify recombinant proteins from culture supernatants. Such assays can be easily and rapidly developed. In this paper, we describe the development and validation of an analytical technique for quantifying HER1 extracellular domain (HER1 ECD) in bioreactor supernatant of HEK 293 …

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  • Development and Validation of a Method for

    Vip3Aa1 quantification in seeds and leaves of transgenic corn plants: Immunological assays have been broadly used for non-purified sample screening and specific quantification of target proteins expressed and purified from transgenic plants (Kumar et al., 2010). As it was mentioned earlier, all this previous purification and characterization ...

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  • Bacillus thuringiensis Vip3Aa1 Expression and

    2020-12-9 · Development of hybrid varieties is one of the strategies to increase the productivity and production of Maize (Zea mays L.) in Sri Lanka. Heterosis is an important aspect to measure the hybrid vigor. Genetic distance is a main factor, which affects on heterosis. Identification of suitable parents using molecular markers gives many advantages over morphological evaluation.

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  • Vicente Vérez Bencomo - CIGB

    2004-3-22 · 2Laboratory of Synthetic Antigens, Faculty of Chemistry, University of Havana, Havana 10400, Cuba 3Fakultat der Chemie, University of Konstanz , Germany ABSTRACT ... series of 3'-sialyllactose derivatives coupled to casein or polyacrylamide. In general, the neoglycoconjugates in- ... the conjugates was determined by the quantification of ...

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  • SciELO - Saúde Pública - HIV-2 antibody detection

    ABSTRACT. INTRODUCTION Differentiating between HIV-1 and HIV-2 infection is the first step to understanding HIV transmission, epidemiology and pathogenesis in geographical areas where both viruses circulate. In Cuba, positive results in mixed HIV-1/2 screening assays are confirmed by HIV-1 Western blot. Indeterminate results constitute the main limitation of this test and HIV-2 infection is ...

    Get Price
  • ˇ˘ ˆ ˝ ’˙ - IAEA

    2001-10-1 · Ms. A.Mendoza, Cuba). The spectrometer provides an opportunity for direct and secondary target excitation. By choosing a proper target and/or filter one can optimise the excitation conditions for selected element(s). A set of different collimators provides the primary photon beam in the range of 1 mm -1 cm in diameter.

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  • Differential proteomic analysis of virus-enriched ...

    2017-8-29 · Additionally, reports from Cuba and Australia have sug-gested that circulating DV may become more virulent through passage in successive hosts during an epidemic [18–21]. As the virus cycle and the virus pathogenicity are strongly linked with the host metabolism, it is assumed that host proteins interacting with virions are a reflec-

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  • Development and Validation of a Method for

    2016-2-11 · Techniques based on high-resolution liquid chromatography are currently widely used to quantify recombinant proteins from culture supernatants. Such assays can be easily and rapidly developed. In this paper, we describe the development and validation of an analytical technique for quantifying HER1 extracellular domain (HER1 ECD) in bioreactor supernatant of HEK 293 …

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  • Protein Electrophoresis and Blotting | Pesquisa de ...

    A guide to polyacrylamide gel electrophoresis and protein detection, including theory, product selection, protocols, and more. Western Blot Doctor Troubleshooting Guide Our self-help troubleshooting guide covers solutions to many common and not-so-common western blotting issues and …

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  • Nutrients | Free Full-Text | Cuban Brown Propolis ...

    Tumor-associated macrophages (TAMs), primarily the M2 phenotype, are involved in the progression and metastasis of colorectal cancer (CRC). Cuban brown propolis (Cp) and its main component Nemorosone (Nem) displays an antiproliferative effect on different cancer cells, including CRC cell lines. However, whether Cp and Nem could exploit its effect on CRC cells by targeting their relationship ...

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  • Gel Analysis Software | Cleaver Scientific

    TotalLab analysis software options are available for quantitative gel analysis following gel documentation. Each software option offers the highest level of automation currently available and guides the user step by step through the analysis process. A user-friendly interface is split into four parts allowing the user to view within a single screen every aspect of gel quantitation, including ...

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  • Microbial inoculants: reviewing the past, discussing

    2019-12-21 · More than one hundred years have passed since the development of the first microbial inoculant for plants. Nowadays, the use of microbial inoculants in agriculture is spread worldwide for different crops and carrying different microorganisms. In the last decades, impressive progress has been achieved in the production, commercialization and use of inoculants.

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  • Bordetella pertussis TECHNIQUE

    2016-12-20 · Ave. 31 e 158 y 190, Cubanacán, Playa, CP 11600, La Habana, Cuba [email protected] ABSTRACT Bordetella pertussis bacterium causes a highly contagious respiratory disease named Pertussis or whooping cough, which represents a major threat to public health. Pt is the main toxin of B. pertussis and one of the most important viru - lence ...

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  • Frontiers | Isolation Method and Characterization of

    2021-6-2 · Protein Quantification of OMVs. A total of 10 11 OMVs were diluted in 4× Laemmli buffer (Bio-Rad Laboratories Inc.) with β-mercaptoethanol (Sigma-Aldrich), denaturated at 95°C for 5 min and loaded onto 7.5% sodium dodecyl sulfate–polyacrylamide gels (SDS-PAGE). After electrophoresis, gels were stained with 25 mL of BlueSafe (NZYTech ...

    Get Price
  • ˇ˘ ˆ ˝ ’˙ - IAEA

    2001-10-1 · Ms. A.Mendoza, Cuba). The spectrometer provides an opportunity for direct and secondary target excitation. By choosing a proper target and/or filter one can optimise the excitation conditions for selected element(s). A set of different collimators provides the primary photon beam in the range of 1 mm -1 cm in diameter.

    Get Price
  • Protein Quantification (Silver Staining) - AbVideo™ -

    2010-3-15 · Protein Quantification (BCA Assay) The bicinchoninic acid assay (BCA Assay) is a biochemical assay for determining the total level of protein in a solution, similar to Bradford protein assay. The total protein concentration is exhibited by a color change of the sample solution from green to purple in proportion to protein concentration, which can then be measured using colorimetric techniques.

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  • Differential proteomic analysis of virus-enriched ...

    2017-8-29 · Additionally, reports from Cuba and Australia have sug-gested that circulating DV may become more virulent through passage in successive hosts during an epidemic [18–21]. As the virus cycle and the virus pathogenicity are strongly linked with the host metabolism, it is assumed that host proteins interacting with virions are a reflec-

    Get Price
  • Complete sucrose hydrolysis by heat-killed

    2014-6-18 · Results. In this study, heat-killed Pichia pastoris cells bearing N-glycosylated BfrA in the periplasmic space were entrapped in calcium alginate beads. The immobilized recombinant yeast showed maximal sucrose hydrolysis at pH 5–7 and 90°C. BfrA was 65% active at 60°C and had no activity loss after incubation without the substrate at this temperature for 15 h.

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  • A Comprehensive Review on the Main Honey

    2021-1-20 · To that end, we’ve compiled lists of the five most-read chemistry articles from each ACS Publications journal in all of 2020, including research, reviews, perspectives, and editorial pieces. These lists were not chosen by the journal’s editors and should not be taken as a “best of” list, but as another perspective on where the chemistry ...

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  • Cuban Sugar Cane Wax Acid and Policosanol Showed

    2019-2-24 · Quantification of oxidized species in cell media revealed that cells treated with oxLDL alone contained 2-fold more MDA than PBS-treated cells. As a control, rHDL-treated cells had 20% less MDA than those treated with oxLDL alone; however, SCWA-rHDL- or PCO-rHDL-treated cells contained 45% or 42% less MDA than cells treated with oxLDL alone. 3.7.

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  • Frontiers | Isolation Method and Characterization of

    2021-6-2 · Protein Quantification of OMVs. A total of 10 11 OMVs were diluted in 4× Laemmli buffer (Bio-Rad Laboratories Inc.) with β-mercaptoethanol (Sigma-Aldrich), denaturated at 95°C for 5 min and loaded onto 7.5% sodium dodecyl sulfate–polyacrylamide gels (SDS-PAGE). After electrophoresis, gels were stained with 25 mL of BlueSafe (NZYTech ...

    Get Price
  • Protein Quantification (Silver Staining) - AbVideo™ -

    2010-3-15 · Protein Quantification (BCA Assay) The bicinchoninic acid assay (BCA Assay) is a biochemical assay for determining the total level of protein in a solution, similar to Bradford protein assay. The total protein concentration is exhibited by a color change of the sample solution from green to purple in proportion to protein concentration, which can then be measured using colorimetric techniques.

    Get Price
  • Protein Electrophoresis: Methods and Protocols |

    2021-7-4 · DNA, RNA and PCR products. The polymerase chain reaction (PCR) allows isolation of DNA fragments from genomic DNA by selective amplification of a specific region of DNA. It has been adapted over the years to allow amplification of RNA samples, as well as quantification of the amount of DNA or RNA in a sample.

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  • DNA, RNA, and PCR Products: Polymerases, Enzymes,

    2012-2-23 · The samples were incubated in another 4 mL of equilibration buffer with 25 mg/mL iodoacetamide for 15 minutes at 22°C with shaking in order to ensure protein S-alkylation. Electrophoresis was performed at 150 V for 2.25 hours, 4°C with precast 8–16% polyacrylamide gels in Tris-glycine buffer (25 mM Tris-HCl, 192 mM glycine, 0.1% SDS, pH 8.3).

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  • Discovery Proteomics and Nonparametric Modeling

    2014-3-20 · miR-566 is over-expressed in glioma cell lines and activates EGFR/Akt signaling. We previously demonstrated that the expression of five microRNAs (miR-181d, miR-518b, miR-524-5p, miR-566 and miR-1227) were correlated with the survival of glioblastoma patients [].Previous studies have clarified the functions of the miR-181 family, miR-518b, miR-524-5p and miR-1227.

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  • MicroRNA-566 activates EGFR signaling and its

    The technique is based on separation of DNA fragments that differ in nucleotide sequences (e.g. species specific) through decreased electrophoretic mobility of partially melted double-stranded DNA amplicons in a polyacrylamide gel containing a linear gradient of DNA denaturants (i.e. a …

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  • Advances in yeast systematics and phylogeny and

    2021-1-20 · To that end, we’ve compiled lists of the five most-read chemistry articles from each ACS Publications journal in all of 2020, including research, reviews, perspectives, and editorial pieces. These lists were not chosen by the journal’s editors and should not be taken as a “best of” list, but as another perspective on where the chemistry ...

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  • The Most-Read ACS Publications Journal Articles of

    2015-11-19 · Therefore, Hcy quantification is included among the first recommended steps for differential diagnosis and management of these disorders. The diagnosis of classic homocystinuria in Cuba is routinely carried out by a qualitative test, which does not have the necessary sensitivity to detect milder Hcy elevations in combined methylmalonic ...

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  • MEDICC Review - Revention and treatment of Breast

    2004-5-28 · In Cuba, ER was first given in 1972 by means of biochemical methods such as dextran-coated charcoal followed by Scatchard plotting 11,12, the isoelectric approach method 13,14,15 and the sucrose-density gradient.

    Get Price
  • Bordetella pertussis TECHNIQUE

    2016-11-7 · Ave. 31 e 158 y 190, Cubanacán, Playa, CP 11600, La Habana, Cuba [email protected] . ABSTRACT. Bordetella pertussis. bacterium causes a highly contagious respiratory disease named Pertussis or whooping cough, which represents a major threat to public health. Pt is the main toxin of . B. pertussis. and one of the most important viru ...

    Get Price
  • Major Carotenoids in Tobacco Laminas Identification

    Major Carotenoids in Tobacco Laminas: Identification and Quantification by HPLC with Photodiode Array Detection Author Creator: Chu, D. Xie, Y. ... Use of the Different Polyacrylamide Gel Electrophoresis (PAGE) Methods in the Characterization of Human Pathogenic Staphylococcus aureus Strains. Berber, I. …

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  • ALTERATIONS OF THE ANTIOXIDANT ENZYME

    2017-6-29 · gel- 4% polyacrylamide and separating gel- 8.5% and 10% for POX and SOD, respectively. Electrophoreses were run with 19 mM Tris-Glycine buffer (pH 8.3) at 25 mA for 2 h at 4 ºC in a BIORAD vertical system. Each lane was loaded with 20 μg protein. The POX isoenzymes on gel were revealed by staining with acetic acid-benzydin solution and hydrogen

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  • Differential Expression of Vibrio vulnificus Capsular ...

    2020-12-18 · Vibrio vulnificus is a human pathogen whose virulence has been associated with the expression of capsular polysaccharide (CPS). Multiple CPS types have been described; however, virulence does not appear to correlate with a particular CPS composition. Reversible-phase variation for opaque and translucent colony morphologies is characterized by changes in CPS expression, as …

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  • (PDF) A rapid and sensitive ELISA to quantify an

    A rapid and sensitive ELISA to quantify an HBsAg specific monoclonal antibody and a plant-derived antibody during their downstream purification process

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  • Altered non-coding RNA expression profile in F 1

    2021-2-18 · Quantification values derived from five technical replicate per biological replicate. Relative quantification was normalized to a synthetic spike-in kanamycin RNA and expression levels were ...

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  • Differential expression of porcine microRNAs in

    2017-10-17 · African swine fever (ASF) is a re-expanding devastating viral disease currently threatening the pig industry worldwide. MicroRNAs are a class of 17–25 nucleotide non- coding RNAs that have been shown to have critical functions in a wide variety of biological processes, such as cell differentiation, cell cycle regulation, carcinogenesis, apoptosis, regulation of immunity as well as in viral ...

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