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quantification polyacrylamide gel electrophoresis application

  • Application of polyacrylamide gel

    A combination of two methods, polyacrylamide gel electrophoresis (PAGE) and neutron activation analysis (NAA), has been applied to solutions containing phosphoproteins for the purpose of protein quantification. The proteins were separated by molecular weight using PAGE, and then the whole gel was activated by neutron bombardment.

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  • Quantification of rat neurophysins by polyacrylamide

    Quantification of rat neurophysins by polyacrylamide gel electrophoresis (PAGE): application to the rat with hereditary hypothalamic diabetes insipidus. Ann N Y Acad Sci. 1975 Feb 21;248:345-64. doi: 10.1111/j.1749-6632.1975.tb34197.x.

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  • Quantification of Polyacrylamide Gel Electrophoresis

    1976-5-1 · Polyacrylamide gel electrophoresis (PAGE) is used widely as a qualitative technique for separation and identification of proteins. The high resolution that is possible with PAGE makes it an extremely powerful tool in the analysis of protein mixtures.

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  • Quantification of rat neurophysins by polyacrylamide

    1975-2-21 · Quantification of rat neurophysins by polyacrylamide gel electrophoresis (PAGE): application to the rat with hereditary hypothalamic diabetes insipidus. Sunde DA, Sokol HW. PMID: 1054551 [PubMed - indexed for MEDLINE] Publication Types: Research Support, U.S. Gov't, P.H.S. MeSH Terms. Animals; Diabetes Insipidus/genetics; Diabetes Insipidus/physiopathology*

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  • Polyacrylamide gel electrophoresis in quantification of ...

    Abstract. We evaluated a method for quantifying high-density lipoprotein cholesterol in plasma, based on electrophoretic migration of the prestained (with Sudan Black III) sample through a discontinuous polyacrylamide++ gel and densitometric integration of the stain associated with each class of lipoprotein. With this method, operations can be ...

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  • Polyacrylamide Gel Electrophoresis | Cleaver Scientific

    Application of polyacrylamide gel as a new membrane . 2018-3-20 · Application of polyacrylamide gel as a new membrane in electromembrane extraction for the quantification of basic drugs in breast milk and wastewater samples - ScienceDirect Journal of Pharmaceutical and Biomedical Analysis Volume 151, 20 March 2018, Pages 178-185

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  • application of polyacrylamide quantification

    The authors present a variant of the technique of sodium dodecylsulfate acrylamide gel electrophoresis (SDS-PAA) reported by Weber and Osborn, for the analysis of urinary proteins. SDS-PAA separates the proteins chiefly according to their molecular radius. SDS-PAA, as compared to acetate cellulose electrophoresis and immunoelectrophoresis, gives ...

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  • [Application of polyacrylamide gel electrophoresis

    1991-9-13 · Polyacrylamide gel electrophoresis (PAGE) and staining of proteins is relatively easy to perform, but quantification of separated proteins is often necessary. It is quite easy to measure stained protein bands with a densitometer, however, it is much more difficult to evaluate the measured absorbance and to obtain reliable quantitative data reflecting the amount of the protein actually present.

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  • Staining and quantification of proteins separated by ...

    2019-3-15 · Native polyacrylamide gel electrophoresis (N-PAGE) is a simple qualitative technology to determine heterogeneity of proteins. Here, we have applied N-PAGE to examine heat-induced aggregation and oligomerization of bovine serum albumin (BSA) and the effects of temperature, caprylic acid, N-acetyl-tryptophan, DNA, arginine and gallic acid.

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  • Quantification of Polyacrylamide Gel

    1976-5-1 · Quantification of Polyacrylamide Gel Electrophoresis for Analysis of Whey Proteins D. F. DARLING and D. W. BUTCHER Dairy Products Section Unilever Research Laboratory Welvvyn, Hefts, England ABSTRACT Polyacrylamide gel electrophoresis of whey proteins has been quantified by standardization of the separation and staining procedure.

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  • application of polyacrylamide quantification

    Application of polyacrylamide gel as a new membrane . 2018-3-20 · Application of polyacrylamide gel as a new membrane in electromembrane extraction for the quantification of basic drugs in breast milk and wastewater samples - ScienceDirect Journal of Pharmaceutical and Biomedical Analysis Volume 151, 20 March 2018, Pages 178-185

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  • QUANTIFICATION OF RAT NEUROPHYSINS BY

    QUANTIFICATION OF RAT NEUROPHYSINS BY POLYACRYLAMIDE GEL ELECTROPHORESIS (PAGE): APPLICATION TO THE RAT WITH HEREDITARY HYPOTHALAMIC DIABETES INSIPIDUS * David A. Sunde, Department of Physiology Dartmouth Medical School Hanover, New Hampshire 03755. Search for more papers by this author.

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  • Quantification of Rat Neurophysins by

    Quantification of Rat Neurophysins by Polyacrylamide Gel Electrophoresis (page): Application to the Rat with Hereditary Hypothalamic Diabetes Insipidus Sunde, David A. Sokol, Hilda Weyl

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  • Application of polyacrylamide gel as a new

    2018-3-20 · Application of polyacrylamide gel as a new membrane in electromembrane extraction for the quantification of basic drugs in breast milk and wastewater samples. ... Electrically assisted liquid-Phase microextraction combined with capillary electrophoresis for quantification of propranolol enantiomers in human body fluids. Chirality, 26 (2014), pp ...

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  • Quantification of Proteins by Staining in

    Dráber, P. (1991) Quantification of proteins in sample buffer for sodium dodecyl sulfate-polyacrylamide gel electrophoresis using colloidal silver. Electrophoresis 12, 453–456. PubMed CrossRef Google Scholar

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  • Polyacrylamide Gel Electrophoresis | Cleaver Scientific

    2019-3-15 · Native polyacrylamide gel electrophoresis (N-PAGE) is a simple qualitative technology to determine heterogeneity of proteins. Here, we have applied N-PAGE to examine heat-induced aggregation and oligomerization of bovine serum albumin (BSA) and the effects of temperature, caprylic acid, N-acetyl-tryptophan, DNA, arginine and gallic acid.Arginine showed marginal protection of BSA …

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  • Application of native polyacrylamide gel

    2019-5-25 · 32P-Postlabeling analysis is a powerful technique to detect DNA adducts. Polyethylenimine−cellulose TLC plates are generally used to separate 32P-labeled adducts using several different buffers. However, separation by TLC is time-consuming and labor-intensive for a large number of DNA samples. To expedite analyses, nondenaturing 30% polyacrylamide gel electrophoresis …

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  • application of polyacrylamide quantification

    Application of polyacrylamide gel as a new membrane . 2018-3-20 · Application of polyacrylamide gel as a new membrane in electromembrane extraction for the quantification of basic drugs in breast milk and wastewater samples - ScienceDirect Journal of Pharmaceutical and Biomedical Analysis Volume 151, 20 March 2018, Pages 178-185

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  • Quantification of Proteins on Polyacrylamide Gels ...

    Abstract. Quantification of proteins is a common challenge. There are various methods described for estimation of the amount of protein present in a sample, for example, amino acid analysis and the bicinchoninic acid (BCA) method (see Chapters 83 and 3).Proteins may be quantified in sample solvent prior to electrophoresis and can provide an estimate of total amount of protein in a sample ...

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  • Staining and quantification of proteins separated by ...

    1991-9-13 · Staining and quantification of proteins separated by polyacrylamide gel electrophoresis. Syrový I(1), Hodný Z. Author information: (1)Institute of Physiology, Czechoslovak Academy of Sciences, Prague. The present review concentrates on techniques for the staining and quantification of proteins separated by polyacrylamide gel electrophoresis.

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  • Quantification of proteins in sample buffer for sodium ...

    Quantification of proteins in sample buffer for sodium dodecyl sulfate‐polyacrylamide gel electrophoresis using colloidal silver. Dr. Pavel Dráber. Corresponding Author. ... A rapid and simple assay for quantification of proteins in sodium dodecyl sulfate‐sample buffer is described. Proteins are bound to a nitrocellulose membrane, stained ...

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  • Histochemical staining and quantification of plant ...

    2005-11-9 · Histochemical staining and quantification of plant mitochondrial respiratory chain complexes using blue‐native polyacrylamide gel electrophoresis Mohammed Sabar Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK

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  • Quantification of muscle mitochondrial oxidative ...

    2005-4-14 · Blue native‐polyacrylamide gel electrophoresis is a powerful technique that enables the separation of intact multi‐subunit complexes. However, positive identification of particular enzymes generally requires further separation in a second dimension on a denaturing polyacrylamide gel.

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  • Spotting and quantification of phosphoproteins

    Laser ablation inductively coupled plasma‐mass spectrometry (ICP‐MS) with 31 P detection has been used for spotting of phosphoproteins after one‐dimensional polyacrylamide gel electrophoresis (1‐D PAGE) and membrane transfer. By analyzing a mixture of myoglobin, α‐casein and reduced fibrinogen it is demonstrated that phosphoproteins are specifically recognized by this method.

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  • Polyacrylamide Gel Electrophoresis (PAGE ...

    Application Of Gel Electrophoresis Gel electrophoresis technique has been widely used in many area in biotechnology includes molecular biology , biochemistry, genetics and forensics . The result of gel electrophoresis is often incorporated with other techniques depends on the type of analysis being carried out and thus provided a large range of ...

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  • quantification polyacrylamide gel electrophoresis in

    Quantification of DNA by agarose gel electrophoresis . quantification of rat neurophysins by polyacrylamide gel electrophoresis (page): application to the rat with hereditary hypothalamic diabetes insipidus * David A. Sunde , Department of Physiology Dartmouth Medical School Hanover, New Hampshire 03755

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  • Quantification of Proteins on Polyacrylamide Gels ...

    Draber, P. (1991) Quantification of proteins in sample buffer for sodium dodecyl sulfate-polyacrylamide gel electrophoresis using colloidal silver. Electrophoresis 12, 453–456. PubMed CrossRef Google Scholar

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  • Histochemical staining and quantification of plant ...

    2005-11-9 · Histochemical staining and quantification of plant mitochondrial respiratory chain complexes using blue‐native polyacrylamide gel electrophoresis Mohammed Sabar Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK

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  • Gel Electrophoresis of Proteins

    2014-6-10 · Gel electrophoresis is a broad subject encompassing many different techniques. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is the most commonly practiced gel electrophoresis technique used for proteins. The method provides an easy way to estimate the number of polypeptides in a sample and thus assess

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  • The principle and Procedure of Polyacrylamide Gel ...

    Abstract. The majority of cardiovascular proteomic investigations reported to date have employed two-dimensional gel electrophoresis (2DE) with immobilized pH gradients to separate the sample proteins, combined with quantitative computer analysis to detect differentially expressed proteins and mass spectrometry technologies to identify proteins of interest.

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  • Two-Dimensional Polyacrylamide Gel Electrophoresis

    2005-12-1 · Functional analysis of the plant mitochondrial respiratory chain has been successfully achieved by a combination of blue-native polyacrylamide gel electrophoresis (BN-PAGE) for separation of the protein complexes, and in-gel histochemical staining of the enzyme activities.

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  • A Novel Gel Electrophoresis Technique for Rapid

    Abstract A method is described for determining the electrophoretic behavior of circulating insulin immunoreactive materials. A polyacrylamide immunoreactive pattern of serum can be determined on as little as 100 μU of insulin immunoreactive material. It is suggested that the utility of the method would be increased if it were coupled to a prior chromatographic separation of proinsulin like ...

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  • quantification polyacrylamide gel electrophoresis in

    Quantification of DNA by agarose gel electrophoresis . quantification of rat neurophysins by polyacrylamide gel electrophoresis (page): application to the rat with hereditary hypothalamic diabetes insipidus * David A. Sunde , Department of Physiology Dartmouth Medical School Hanover, New Hampshire 03755

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  • Quantification of polyacrylamide gel bands by

    1. Anal Biochem. 1983 Sep;133(2):476-81. Quantification of polyacrylamide gel bands by digital image processing. Stanley KK, Pitt TJ. A method of quantifying bands on polyacrylamide gels based on image processing of digitized photographic negatives is presented.

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  • Chemical quantification of melanophore stimulating ...

    1970-2-22 · 1. Life Sci. 1970 Feb 22;9(4):215-28. Chemical quantification of melanophore stimulating substances using polyacrylamide gel disc electrophoresis.

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  • Location and quantification of metal ions in

    Location and quantification of metal ions in enzymes combining polyacrylamide gel electrophoresis and particle-induced X-ray emission. Zoltán Szökefalvi-Nagy 1, Csaba Bagyinka 2, István Demeter 1, Kornél L. Kovács 2 & Huen Le Quynh 2 Biological Trace Element Research volume 26, pages 93–101 (1990)Cite this article

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  • Quantification of proteins in sample buffer for

    Quantification of proteins in sample buffer for sodium dodecyl sulfate-polyacrylamide gel electrophoresis using colloidal silver. Dráber P(1). Author information: (1)Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague. A rapid and simple assay for quantification of proteins in sodium dodecyl sulfate-sample buffer is ...

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  • A novel gel electrophoresis technique for rapid

    2021-2-18 · Polyacrylamide gel electrophoresis enables high-resolution separation of proteins extracted from biological samples, but it requires more than one day of pretreatment to recover the separated ...

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  • [PDF] Polyacrylamide gel electrophoresis of

    This report describes a new formulation of polyacrylamide gel electrophoresis of fluorophore‐labeled saccharides (PAGEFS) for the analysis of hyaluronan (HA) and chondroitin sulfate (CS) Δ‐disaccharides. PAGEFS relies on derivatization of reducing ends of HA‐ and the variously sulfated CS‐derived Δ‐disaccharides with 2‐aminoacridone (AMAC), followed by electrophoresis under ...

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  • The application of two‐dimensional polyacrylamide

    This paper reviews the application of two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) for studying microbial molecular epidemiology. A small format 2-D PAGE system is described for locating protein markers in group B coxsackie viruses (CVB) and Haemophilus infuenzae isolates.

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  • Polyacrylamide Gel Electrophoresis (PAGE ...

    Abstract A method is described for determining the electrophoretic behavior of circulating insulin immunoreactive materials. A polyacrylamide immunoreactive pattern of serum can be determined on as little as 100 μU of insulin immunoreactive material. It is suggested that the utility of the method would be increased if it were coupled to a prior chromatographic separation of proinsulin like ...

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  • quantification cationic polyacrylamide gel

    Protein quantification and electrophoresis in aqueous ... Two-Dimensional Polyacrylamide Gel Electrophoresis – A Practical Perspective 111 7.4 Concluding remarks In spite of above mentioned limiting factors, it has been shown that even a 25% change in protein quantity can be reliably detected in most of matched spots by using ruthenium fluorescent dye provided that one can control ...

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  • A novel gel electrophoresis technique for rapid biomarker ...

    2021-7-4 · Polyacrylamide gel electrophoresis enables high- ... MS-based quantification of protein biomarkers is ... via Dissolvable Polyacrylamide Gel Electrophoresis and Its Application …

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  • Location and quantification of metal ions in enzymes ...

    Location and quantification of metal ions in enzymes combining polyacrylamide gel electrophoresis and particle-induced X-ray emission. Zoltán Szökefalvi-Nagy 1, Csaba Bagyinka 2, István Demeter 1, Kornél L. Kovács 2 & Huen Le Quynh 2 Biological Trace Element Research volume 26, pages 93–101 (1990)Cite this article

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  • Polyacrylamide Gel Electrophoresis of RNA

    There are two common types of gel: polyacrylamide and agarose. For most applications, denaturing acrylamide gels are most appropriate. These gels are extremely versatile and can resolve RNAs from ~600 to ≤20 nucleotides (nt). In certain circumstances, e.g., resolving different conformers of RNAs or RNA-protein complexes, native gels are ...

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  • Polyacrylamide gel electrophoresis of reducing

    Polyacrylamide gel electrophoresis of reducing saccharides labeled with the fluorophore 8-aminonaphthalene-1,3,6-trisulphonic acid: Application to the enzymological structural analysis of oligosaccharides. Dr. Peter Jackson, Corresponding Author. Astromed Limited, Innovation Centre, Cambridge Science Park, Milton Road, Cambridge ...

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  • A Novel Gel Electrophoresis Technique for Rapid

    2004-1-1 · So far, the application of two‐dimensional polyacrylamide gel electrophoresis (2D‐PAGE) and downstream analyses has been limited to pure cultures of different microorganisms ( …

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  • The application of two‐dimensional polyacrylamide

    2021-2-18 · Polyacrylamide gel electrophoresis enables high-resolution separation of proteins extracted from biological samples, but it requires more than one day of pretreatment to recover the separated ...

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  • A novel gel electrophoresis technique for rapid

    2005-12-1 · Functional analysis of the plant mitochondrial respiratory chain has been successfully achieved by a combination of blue-native polyacrylamide gel electrophoresis (BN-PAGE) for separation of the protein complexes, and in-gel histochemical staining of the enzyme activities.

    Get Price
  • quantification polyacrylamide gel electrophoresis in

    Quantification of DNA by agarose gel electrophoresis . quantification of rat neurophysins by polyacrylamide gel electrophoresis (page): application to the rat with hereditary hypothalamic diabetes insipidus * David A. Sunde , Department of Physiology Dartmouth Medical School Hanover, New Hampshire 03755

    Get Price
  • An SDS-PAGE based method for the quantification

    Quantification of proteins in sample buffer for sodium dodecyl sulfate-polyacrylamide gel electrophoresis using colloidal silver. Dráber P(1). Author information: (1)Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague. A rapid and simple assay for quantification of proteins in sodium dodecyl sulfate-sample buffer is ...

    Get Price
  • Quantification of proteins in sample buffer for

    1991-9-13 · Staining and quantification of proteins separated by polyacrylamide gel electrophoresis. Syrový I(1), Hodný Z. Author information: (1)Institute of Physiology, Czechoslovak Academy of Sciences, Prague. The present review concentrates on techniques for the staining and quantification of proteins separated by polyacrylamide gel electrophoresis.

    Get Price
  • Staining and quantification of proteins separated by ...

    The electrophoresis of histones in polyacrylamide gel and their quantitative determination. Johns EW. Biochem J, 104(1):78-82, 01 Jul 1967 Cited by: 157 articles | PMID: 6035526 | …

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  • A simple method for the application of two samples

    Corpus ID: 73603912. Detection and quantification of phytic acid in black pepper variety Panniyur-1 using Polyacrylamide Gel Electrophoresis @article{Giridhari2017DetectionAQ, title={Detection and quantification of phytic acid in black pepper variety Panniyur-1 using Polyacrylamide Gel Electrophoresis}, author={A. C. Giridhari and R. Sujatha and K. P. Deeshma}, journal={Journal of Tropical ...

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  • Detection and quantification of phytic acid in black ...

    2005-11-9 · Histochemical staining and quantification of plant mitochondrial respiratory chain complexes using blue‐native polyacrylamide gel electrophoresis Mohammed Sabar Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK

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  • Histochemical staining and quantification of plant ...

    2007-8-23 · Resolve 10 μl of the PCR products, mixed with 2 μl of 6 × loading dye, by electrophoresis on a polyacrylamide gel (usually 8%) or agarose gel (2%) appropriate for the expected PCR product size ...

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  • The principle and method of polyacrylamide gel ...

    Polyacrylamide gel electrophoresis (PAGE) provides a versatile, gentle, high resolution method for fractionation and physical-chemical characterization of molecules on the basis of size, conformation, and net charge. The polymerization reaction can be rigorously controlled to provide uniform gels of reproducible, measurable pore size over a wide range.

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