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    Quantitative polyacrylamide gel electrophoresis . Quantitative polyacrylamide gel electrophoresis for cationic proteins from polymorphonuclear (PMN) leukocytes. Sajnani AN, Movat HZ, Ranadive NS. PMID: 4142521 [PubMed - indexed for MEDLINE] MeSH Terms.

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    Quantitative polyacrylamide gel electrophoresis for cationic proteins from polymorphonuclear (PMN) leukocytes. Sajnani AN, Movat HZ, Ranadive NS. PMID: 4142521 [PubMed - indexed for MEDLINE] MeSH Terms. Animals; Densitometry; Electrophoresis, Polyacrylamide Gel/methods* Leukocytes/analysis* Proteins/analysis* Rabbits; Staining and Labeling; Substances. Proteins

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    1974-9-1 · Quantitative polyacrylamide gel electrophoresis for cationic proteins from polymorphonuclear (PMN) leukocytes.

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    For example, quantitative preparative native continuous polyacrylamide gel electrophoresis (QPNC-PAGE) is a method for separating native metalloproteins in complex biological matrices. In order to ensure maximum reproducibility in gel pore size and to obtain a fully polymerized and non-restrictive large pore gel for a PAGE run, the polyacrylamide gel is polymerized for a time period …

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    2013-3-1 · Non-denaturing polyacrylamide gel electrophoresis 35 Introduction 35 ... Tricine SDS-polyacrylamide gel electrophoresis 46 Use of cationic detergents for PAGE 47 Acknowledgements 49 References 50 2. ... Quantitative analysis of electrophoretically separated proteins 85 References 86 3.

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    The isolated material was homogeneous as determined by cationic and sodium dodecyl sulfate/polyacrylamide gel electrophoresis, ultracentrifugation, amino acid and amino-terminal analyses, and immunoelectrophoretic analysis. The one-step purification procedure yielded a 1370-fold increase in specific activity.

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    Polyacrylamide gel electrophoresis is conducted utilizing a published procedure [32].Samples of approximately 50 µg of the antibody solution were subjected to electrophoresis.The buffer solution was of pH 8.3 and consisted of 0.005 M Tris and 0.04 M glycine.Electrophoresis was conducted at a constant current of 2.5 ma/gel for periods of 4 to 6 hrs.

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    Quantitative Assessment of Chimeraplast Stability in Biological Fluids by Polyacrylamide Gel Electrophoresis and Laser-Assisted Fluorescence Analysis. David de Semir 1, Anna Avinyó 1, Sara Larriba 1, Virginia Nunes 1, Teresa Casals 1, Xavier Estivill 1 & Josep M. Aran 1 Pharmaceutical Research volume 19, pages 914–918 (2002)Cite this article

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    2019-12-12 · To realize efficient homogeneous electrophoretic immunoassays, we introduce discontinuous polyacrylamide gels that enable quantitative assay completion in separation lengths as short as 350 μm in <10 s. The discontinuous cross-linked gels reduce the required electrophoretic separation lengths and thereby significantly reduce the required applied …

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    Polyacrylamide (12.5 %) gel slab electrophoresis in 0.1% sodium dodecyl sulphate was carried out as described by Laemmli (9). Analytical IEF in the pH range 2.5–7.0 was performed on 5.0 % acrylamide gel slab using a Multiphor II electrophoresis system (Pharmacia Biotech) following the manufacturer's instructions. Non-denaturing.

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    1972-5-1 · Quantitative polyacrylamide gel electrophoresis for cationic proteins from polymorphonuclear (PMN) leukocytes. Sajnani AN, Movat HZ, Ranadive NS. Tex Rep Biol Med, 32(3-4):725-733, 01 Sep 1974 Cited by: 0 articles | PMID: 4142521

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    SDS-PAGE (sodium dodecyl sulfate–polyacrylamide gel electrophoresis) is a variant of polyacrylamide gel electrophoresis, an analytical method in biochemistry for the separation of charged molecules in mixtures by their molecular masses in an electric field. These can be run in either agarose or polyacrylamide gels. Polyacrylamide is also often used in molecular biology …

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    Article Title: VELCRO-IP RNA-seq explores ribosome expansion segment function in translation genome-wide Article Snippet: After RNase A elution, the beads were extracted with 30 µl 2x SDS sample buffer, 10 µl of which were analyzed by SDS-PAGE and WB. ..For qualitative assessment of binding and elution efficiencies, an RNA fraction at each step was analysed by 4% polyacrylamide…

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    The isolated material was homogeneous as determined by cationic and sodium dodecyl sulfate/polyacrylamide gel electrophoresis, ultracentrifugation, amino acid and amino-terminal analyses, and immunoelectrophoretic analysis. The one-step purification procedure yielded a 1370-fold increase in specific activity.

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  • Polyacrylamide Gel Electrophoresis - an overview ...

    Polyacrylamide gel electrophoresis is conducted utilizing a published procedure [32].Samples of approximately 50 µg of the antibody solution were subjected to electrophoresis.The buffer solution was of pH 8.3 and consisted of 0.005 M Tris and 0.04 M glycine.Electrophoresis was conducted at a constant current of 2.5 ma/gel for periods of 4 to 6 hrs.

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    Quantitative Assessment of Chimeraplast Stability in Biological Fluids by Polyacrylamide Gel Electrophoresis and Laser-Assisted Fluorescence Analysis. David de Semir 1, Anna Avinyó 1, Sara Larriba 1, Virginia Nunes 1, Teresa Casals 1, Xavier Estivill 1 & Josep M. Aran 1 Pharmaceutical Research volume 19, pages 914–918 (2002)Cite this article

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    anionic nonionic flocculant polymer pam . 1996-1-1 · The coupling of IEF with polyacrylamide gel electrophoresis in the presence of the anionic detergent sodium dodecyl sulfate (SDS-PAGE) in the second dimension resulted in a 2D method that separated proteins according to two independent parameters, i.e., charge and size.

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    Polyacrylamide Electrophoresis Gel, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

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    1972-5-1 · Quantitative polyacrylamide gel electrophoresis for cationic proteins from polymorphonuclear (PMN) leukocytes. Sajnani AN, Movat HZ, Ranadive NS. Tex Rep Biol Med, 32(3-4):725-733, 01 Sep 1974 Cited by: 0 articles | PMID: 4142521

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    Article Title: VELCRO-IP RNA-seq explores ribosome expansion segment function in translation genome-wide Article Snippet: After RNase A elution, the beads were extracted with 30 µl 2x SDS sample buffer, 10 µl of which were analyzed by SDS-PAGE and WB. ..For qualitative assessment of binding and elution efficiencies, an RNA fraction at each step was analysed by 4% polyacrylamide…

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  • Polyacrylamide gel electrophoresis - Hyperleap

    SDS-PAGE (sodium dodecyl sulfate–polyacrylamide gel electrophoresis) is a variant of polyacrylamide gel electrophoresis, an analytical method in biochemistry for the separation of charged molecules in mixtures by their molecular masses in an electric field. These can be run in either agarose or polyacrylamide gels. Polyacrylamide is also often used in molecular biology …

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  • Polyacrylamide Gel Electrophoresis - an overview ...

    Polyacrylamide gel electrophoresis is conducted utilizing a published procedure [32].Samples of approximately 50 µg of the antibody solution were subjected to electrophoresis.The buffer solution was of pH 8.3 and consisted of 0.005 M Tris and 0.04 M glycine.Electrophoresis was conducted at a constant current of 2.5 ma/gel for periods of 4 to 6 hrs.

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  • Quantitative Assessment of Chimeraplast Stability in ...

    Quantitative Assessment of Chimeraplast Stability in Biological Fluids by Polyacrylamide Gel Electrophoresis and Laser-Assisted Fluorescence Analysis. David de Semir 1, Anna Avinyó 1, Sara Larriba 1, Virginia Nunes 1, Teresa Casals 1, Xavier Estivill 1 & Josep M. Aran 1 Pharmaceutical Research volume 19, pages 914–918 (2002)Cite this article

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    Nicole Mai Wellcome Trust Centre for Mitochondrial Research, Newcastle University. Cleaver Scientific manufactured us 10 x custom made electrophoresis tanks for running our Gene Read gels. The tanks were made to the exact specification we had requested, and we are extremely pleased with the finished product.

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    7 Two-Dimensional Polyacrylamide Gel Electrophoresis – A Practical Perspective Sameh Magdeldin1,2, Ying Zhang1, Bo Xu1, Yutaka Yoshida1 and Tadashi Yamamoto1 1Department of Structural Pathology, Institute of Nephrology, Graduate School of Medical and Dental Sciences, Niigata University, 2Department of Physiology, Faculty of Veterinary Medicine, Suez Canal …

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