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quantitative polyacrylamide gel electrophoresis in argentina

  • quantitative nonionic polyacrylamide gel

    [Qualitative and quantitative composition of protein fractions in the serum of rats based on the data of polyacrylamide gel electrophoresis]. [Article in Russian] Pandakova VN, Tishchenko EG, Takhtamysheva NA. The protein spectrum of rat blood serum was studied by disc-electrophoresis in polyacrylamide gel. Get Price

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  • Quantitative electrophoresis in polyacrylamide

    1972-1-19 · Gels which contain too much bisacrylamide have a milky-opaque color. Thus, a milky gel is a good indication that excessive amounts of bisacrylamide are present. A 5 % gel containing 25o mg % of bisacrylamide shows a faint tinge of milky color. A 2.5 % gel containing 645 mg of bisacrylamide is milky in color and very …

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  • Quantitative polyacrylamide gel electrophoresis

    1974-9-1 · [Quantitative determination of serum proteins following their separation by disc-electrophoresis in polyacrylamide gel (author's transl)] Strauch L, Tympner KD. Z Klin Chem Klin Biochem, 9(5):405-410, …

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  • The electrophoresis of histones in polyacrylamide

    The electrophoresis of histones in polyacrylamide gel and their quantitative determination. Johns EW. 1. A new method has been devised for the separation of the histone fractions of calf thymus by electrophoresis in polyacrylamide gel at pH2.4. 2.

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  • CHARACTERIZATION OF BOTHROPS VENOMS OF

    2019-3-11 · ammodytoides, B. jararaca and B. jararacussu of Argentina by SDS-Polyacrylamide gel electrophoresis was carried out. Venoms used in this study came from animals (includ-ing juvenile) which live in different geographical zones. Special care was taken to not have cross-contamination of samples with blood and tissue …

    Get Price
  • Polyacrylamide Gel Electrophoresis | Cleaver

    One-dimensional polyacrylamide gel electrophoresis followed by nanocapillary liquid chromatography coupled with mass spectrometry was used to analyze proteins isolated from Staphylococcus aureus UAMS-1 after 3, 6, 12, and 24 h of in vitro growth. Protein abundance was determined using a quantitative …

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  • Relative quantitative comparisons of the

    However, quantitative proteomics using 2D gels is critically dependent on the performances of the protein detection methods used after the electrophoretic separations. This chapter therefore examines critically the various detection methods (radioactivity, dyes, fluorescence, and silver) as well as the data analysis issues …

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  • The whereabouts of 2D gels in quantitative

    In general, electrophoresis is a separation technique based on the migration of charged molecules in response to an electric field, toward the electrode of opposite charge. It is performed mainly in polyacrylamide gels. The most common polyacrylamide gel electrophoresis (PAGE) technique employs the detergent …

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  • SDS-PAGE | Coriolis Pharma

    2002-7-23 · Abstract The characteristics of protein detection and quantitation with SYPRO Ruby protein gel stain in one- and two-dimensional polyacrylamide gels were evaluated. Sodium dodecyl sulfate-polyacryl...

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  • Quantitative polyacrylamide gel electrophoresis

    1977-4-25 · A preparation of human pituitary somatotropin examined in quantitative polyacrylamide gel electrophoresis is conformationally compact. The derived mol…

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  • Electrophoresis of DNA in agarose gels,

    This review describes the electrophoresis of curved and normal DNA molecules in agarose gels, polyacrylamide gels and in free solution. These studies were undertaken to clarify why curved DNA molecules migrate anomalously slowly in polyacrylamide gels but not in agarose gels. Two milestone papers ar …

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  • Sedimentation Equilibrium-Quantitative

    1993-12-1 · Following incubation, 20 μl of each aliquot was applied to individual lanes of a polyacrylamide gel. Following electrophoresis, proteins on the gel were stained with Coomassie blue R-250. The pattern of stained bands on the gel was digitized and the resulting image file analyzed to yield relative intensities of individual bands.

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  • The principle and method of SDS

    Polymerized acrylamide (polyacrylamide) forms a mesh-like matrix suitable for the separation of proteins of typical size. The strength of the gel allows easy handling. Polyacrylamide gel electrophoresis of SDS-treated proteins allows researchers to separate proteins based on their length in an easy, inexpensive, and relatively …

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  • Nuclease detection in SDS-polyacrylamide gel

    2021-6-23 · @article{osti_7107068, title = {Nuclease detection in SDS-polyacrylamide gel electrophoresis}, author = {Rosenthal, A L and Lacks, S A}, abstractNote = {Nucleases can be revealed, following their electrophoretic separation in sodium dodecyl sulfate (SDS)-polyacrylamide gels containing DNA or RNA, by …

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  • Factors affecting quantitation of DNA bands in

    Quantitation of DNA bands separated in polyacrylamide or agarose gels was tested under a variety of conditions to examine key factors contributing to the ability to obtain quantitative data. Variations tested included comparison of different fluorescent stains (ethidium bromide and GelStar stain), a …

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  • Quantitative Detection of Inositol

    Quantitative Detection of Inositol Hexakisphosphate (InsP6) in Crop Plants Using Polyacrylamide Gel Electrophoresis (PAGE) () Mohammad Alimohammadi , Nawab Ali , Mariya Khodakovskaya Department of Applied Science, University of Arkansas at Little Rock, Little Rock, USA .

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  • [PDF] Polyacrylamide Gel Electrophoresis |

    Polyacrylamide gel electrophoresis (PAGE) provides a versatile, gentle, high resolution method for fractionation and physical-chemical characterization of molecules on the basis of size, conformation, and net charge. The polymerization reaction can be rigorously controlled to provide uniform gels of reproducible, …

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  • The electrophoresis of histones in

    1. A new method has been devised for the separation of the histone fractions of calf thymus by electrophoresis in polyacrylamide gel at pH2.4. 2. The fractions have been characterized by their relative mobilities with respect to a marker protein, bovine plasma albumin. 3.

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  • Denaturing Polyacrylamide Gel Electrophoresis

    2019-2-5 · Denaturing Polyacrylamide Gel Electrophoresis APPENDIX 3B Thin polyacrylamide gels that contain a high concentration of urea as a denaturant are capable of resolving short ( <500 nucleotides) single-stranded fragments of DNA or RNA that differ in length by as little as one nucleotide. Such gels are uniquely …

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  • 12 nonionic polyacrylamide gel in argentina

    Coating of pre-cast electrophoresis slab gels - Bio-Rad ... What is claimed is: 1. A pre-cast polyacrylamide slab gel for use in slab gel electrophopresis, said pre-cast gel comprising: a pair of chemically inert, transparent plates of a member selected from the group consisting of glass and polystyrene-acrylonitrile blend with inner …

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  • Quantitative polyacrylamide gel electrophoresis

    1974-9-1 · [Quantitative determination of serum proteins following their separation by disc-electrophoresis in polyacrylamide gel (author's transl)] Strauch L, Tympner KD. Z Klin Chem Klin Biochem, 9(5):405-410, …

    Get Price
  • Polyacrylamide Gel Electrophoresis | Cleaver

    For these purposes, a new state-of-the-art gel electrophoresis [quantitative preparative native continuous polyacrylamide gel electrophoresis procedure (QPNC-PAGE)] combined with biological mass and NMR spectrometries might essentially contribute to provide fundamental insights into the metabolisms of important …

    Get Price
  • Important Contributions of a New Quantitative

    1. A new method has been devised for the separation of the histone fractions of calf thymus by electrophoresis in polyacrylamide gel at pH2.4. 2. The fractions have been characterized by their relative mobilities with respect to a marker protein, bovine plasma albumin. 3. A method has been developed for the quantitative …

    Get Price
  • The Electrophoresis of Histones in Polyacrylamide

    the successful practice of the electrophoretic technique, by using polyacrylamide gel in shrimps. Keywords: Polyacrylamide electrophoresis gel, Enzyme, Shrimps 1. Introduction Enzyme electrophoresis is a method for studying genetic polymorphism at a number of enzyme loci enables us to characterize a strain or a species …

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  • Enzyme Electrophoresis in Shrimps by Polyacrylamide

    Polyacrylamide Gel Electrophoresis. Michael R. Green and Joseph Sambrook; Abstract. Cross-linked chains of polyacrylamide can be used as electrically neutral gels to separate double-stranded DNA fragments according to size and single-stranded DNAs according to size and conformation. Polyacrylamide gels have the …

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  • Polyacrylamide Gel Electrophoresis - CSH Protocols

    Polymerized acrylamide (polyacrylamide) forms a mesh-like matrix suitable for the separation of proteins of typical size. The strength of the gel allows easy handling. Polyacrylamide gel electrophoresis of SDS-treated proteins allows researchers to separate proteins based on their length in an easy, inexpensive, and relatively …

    Get Price
  • The principle and method of SDS-polyacrylamide

    Quantitative Detection of Inositol Hexakisphosphate (InsP6) in Crop Plants Using Polyacrylamide Gel Electrophoresis (PAGE) () Mohammad Alimohammadi , Nawab Ali , Mariya Khodakovskaya Department of Applied Science, University of Arkansas at Little Rock, Little Rock, USA .

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  • Quantitative Detection of Inositol

    2021-3-9 · kDawere isolated by using a combination of GPC and quantitative preparative native continuous polyacrylamide gel electrophoresis (QPNC-PAGE). Plant supernatant was first subjected to GPC and the high molecular mass Cdproteins in a GPC fraction of this plant were isolated by QPNC-PAGE. As a result, the …

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  • 12 nonionic polyacrylamide gel in argentina

    Coating of pre-cast electrophoresis slab gels - Bio-Rad ... What is claimed is: 1. A pre-cast polyacrylamide slab gel for use in slab gel electrophopresis, said pre-cast gel comprising: a pair of chemically inert, transparent plates of a member selected from the group consisting of glass and polystyrene-acrylonitrile blend with inner …

    Get Price
  • Quantitative polyacrylamide gel electrophoresis

    1974-9-1 · [Quantitative determination of serum proteins following their separation by disc-electrophoresis in polyacrylamide gel (author's transl)] Strauch L, Tympner KD. Z Klin Chem Klin Biochem, 9(5):405-410, …

    Get Price
  • The principle and method of SDS

    Polymerized acrylamide (polyacrylamide) forms a mesh-like matrix suitable for the separation of proteins of typical size. The strength of the gel allows easy handling. Polyacrylamide gel electrophoresis of SDS-treated proteins allows researchers to separate proteins based on their length in an easy, inexpensive, and relatively …

    Get Price
  • Important Contributions of a New Quantitative

    For these purposes, a new state-of-the-art gel electrophoresis [quantitative preparative native continuous polyacrylamide gel electrophoresis procedure (QPNC-PAGE)] combined with biological mass and NMR spectrometries might essentially contribute to provide fundamental insights into the metabolisms of important …

    Get Price
  • Quantitative Detection of Inositol

    Quantitative Detection of Inositol Hexakisphosphate (InsP6) in Crop Plants Using Polyacrylamide Gel Electrophoresis (PAGE) () Mohammad Alimohammadi , Nawab Ali , Mariya Khodakovskaya Department of Applied Science, University of Arkansas at Little Rock, Little Rock, USA .

    Get Price
  • Polyacrylamide gel electrophoresis - Hyperleap

    SDS-PAGE (sodium dodecyl sulfate–polyacrylamide gel electrophoresis) is a variant of polyacrylamide gel electrophoresis, an analytical method in biochemistry for the separation of charged molecules in mixtures by their molecular masses in an electric field. These can be run in either agarose or polyacrylamide gels. Polyacrylamide is …

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  • Two-Dimensional Polyacrylamide Gel

    2018-9-25 · Two-Dimensional Polyacrylamide Gel Electrophoresis A Practical Perspective 95 The 2-D electrophoresis, especially IEF in the first dimension, is very sensitive to many interfering compounds including lipids, nucleic acids, and small ionic molecules. These contaminants can be eliminated by additional steps such as …

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  • A Guide to Polyacrylamide Gel Electrophoresis and

    2018-3-5 · Polyacrylamide Gel Electrophoresis (PAGE) When electrophoresis is performed in acrylamide or agarose gels, the gel serves as a size-selective sieve during separation. As proteins move through a gel in response to an electric field, the gel’s pore structure allows smaller proteins to travel more rapidly than larger …

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  • Global Polyacrylamide Gel Electrophoresis

    Global Polyacrylamide Gel Electrophoresis (PAGE) Market Overview The Global Polyacrylamide Gel Electrophoresis (PAGE) Market is growing at a faster pa...

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  • (PDF) Quantitative assessment of chimeraplast

    Quantitative assessment of chimeraplast stability in biological fluids by polyacrylamide gel electrophoresis and laser-assisted fluorescence analysis. Pharmaceutical Research, 2002. Sara Larriba. Teresa Casals. J. Aran. David Semir. Sara Larriba. Teresa Casals. J. Aran. David Semir. Download PDF.

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  • 12 nonionic polyacrylamide gel in argentina

    Coating of pre-cast electrophoresis slab gels - Bio-Rad ... What is claimed is: 1. A pre-cast polyacrylamide slab gel for use in slab gel electrophopresis, said pre-cast gel comprising: a pair of chemically inert, transparent plates of a member selected from the group consisting of glass and polystyrene-acrylonitrile blend with inner …

    Get Price
  • Polyacrylamide gel electrophoretic methods in the ...

    1995-4-1 · Polyacrylamide gel electrophoresis plays a major role in analyzing the function of muscle structural proteins. This review describes one- and two-dimensional gel electrophoretic methods for qualitative and quantitative investigation of the muscle proteins, with special emphasis on determination of protein …

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  • Polyacrylamide Gel Electrophoresis | Cleaver

    2019-3-11 · ammodytoides, B. jararaca and B. jararacussu of Argentina by SDS-Polyacrylamide gel electrophoresis was carried out. Venoms used in this study came from animals (includ-ing juvenile) which live in different geographical zones. Special care was taken to not have cross-contamination of samples with blood and tissue …

    Get Price
  • CHARACTERIZATION OF BOTHROPS VENOMS OF

    1. A new method has been devised for the separation of the histone fractions of calf thymus by electrophoresis in polyacrylamide gel at pH2.4. 2. The fractions have been characterized by their relative mobilities with respect to a marker protein, bovine plasma albumin. 3. A method has been developed for the quantitative …

    Get Price
  • The Electrophoresis of Histones in Polyacrylamide

    2008-4-8 · QPNC-PAGE, or quantitative preparative native continuous polyacrylamide gel electrophoresis, is a high-resolution technique applied in biochemistry and bioinorganic chemistry to separate proteins ...

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  • Quantitative Preparative Native Continuous

    Quantitative assessment of chimeraplast stability in biological fluids by polyacrylamide gel electrophoresis and laser-assisted fluorescence analysis. Pharmaceutical Research, 2002. Sara Larriba. Teresa Casals. J. Aran. David Semir. Sara Larriba. Teresa Casals. J. Aran. David Semir. Download PDF.

    Get Price
  • (PDF) Quantitative assessment of chimeraplast

    Method for the quantitative determination of the purity and activity of crystalline insulin by use of electrophoresis in polyacrylamide gel. P. A. Babich 1, T. A. Aslanova 1 & L. I. Nurgalieva 1 Pharmaceutical Chemistry Journal volume 10, pages 694–696 (1976)Cite this article

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  • The principle and method of polyacrylamide gel ...

    Global Polyacrylamide Gel Electrophoresis (PAGE) Market Overview The Global Polyacrylamide Gel Electrophoresis (PAGE) Market is growing at a faster pa...

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  • 12 nonionic polyacrylamide gel in argentina

    Coating of pre-cast electrophoresis slab gels - Bio-Rad ... What is claimed is: 1. A pre-cast polyacrylamide slab gel for use in slab gel electrophopresis, said pre-cast gel comprising: a pair of chemically inert, transparent plates of a member selected from the group consisting of glass and polystyrene-acrylonitrile blend with inner …

    Get Price
  • Quantitative Preparative Native Continuous

    2008-4-8 · QPNC-PAGE, or quantitative preparative native continuous polyacrylamide gel electrophoresis, is a high-resolution technique applied in biochemistry and bioinorganic chemistry to separate proteins ...

    Get Price
  • The principle and method of polyacrylamide gel ...

    2019-3-11 · ammodytoides, B. jararaca and B. jararacussu of Argentina by SDS-Polyacrylamide gel electrophoresis was carried out. Venoms used in this study came from animals (includ-ing juvenile) which live in different geographical zones. Special care was taken to not have cross-contamination of samples with blood and tissue …

    Get Price
  • CHARACTERIZATION OF BOTHROPS VENOMS OF

    2020-4-4 · Title: Important Contributions of a New Quantitative Preparative Native Continuous Polyacrylamide Gel Electrophoresis (QPNC-PAGE) Procedure for Elucidating Metal Cofactor Metabolisms in Protein-Misfolding Diseases - A Theory VOLUME: 13 ISSUE: 5 Author(s):Bernd Kastenholz Affiliation:Institute for …

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  • Important Contributions of a New Quantitative

    Polyacrylamide gel electrophoresis has the advantage over standard agarose gel electrophoresis that improved separation is obtained and the sample is further concentrated when discontinuous buffer systems are used. A combination of polyacrylamide gel electrophoresis with antigen-antibody electrophoresis has …

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  • Identification of Human Serum Proteins by

    Quantitative Detection of Inositol Hexakisphosphate (InsP6) in Crop Plants Using Polyacrylamide Gel Electrophoresis (PAGE) 2013. Mohammad Alimohammadi. Mariya Khodakovskaya. Mohammad Alimohammadi. Mariya Khodakovskaya. Download PDF. Download Full PDF Package. This paper. A short summary of this paper.

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  • (PDF) Quantitative Detection of Inositol

    One-dimensional polyacrylamide gel electrophoresis followed by nanocapillary liquid chromatography coupled with mass spectrometry was used to analyze proteins isolated from Staphylococcus aureus UAMS-1 after 3, 6, 12, and 24 h of in vitro growth. Protein abundance was determined using a quantitative value termed …

    Get Price
  • Relative Quantitative Comparisons of the

    2003-9-1 · Microsatellite DNA markers are widely used in genetic research. Their use, however, can be costly and throughput is sometimes limited. The objective of this paper is to introduce a simple, low-cost, high-throughput system that detects amplification products from microsatellite markers by nondenaturing polyacrylamide gel electrophoresis.

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  • The principle and method of polyacrylamide gel ...

    2014-6-10 · Gel electrophoresis is a broad subject encompassing many different techniques. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is the most commonly practiced gel electrophoresis technique used for proteins. The method provides an easy way to estimate the number of polypeptides in a sample …

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  • Gel Electrophoresis of Proteins

    The quantitative measurement of whey proteins using polyacrylamide-gel electrophoresis - Volume 43 Issue 2. Skip to main content Accessibility help We use cookies to distinguish you from other users and to provide you with a better experience on our websites.

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  • The quantitative measurement of whey proteins

    Polyacrylamide gel electrophoresis, which enables high-resolution separation of proteome prior to top-down mass spectrometry, is a potentially promising technique. However, the lack of an effective approach to recover the separated proteins from the gel after electrophoresis has prevented its application in top-down proteome …

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  • Takemori Lab - Mass Spectrometry &

    2008-9-9 · Gel Electrophoresis Reiner Westermeier, Amersham Biosciences Europe GmbH, Freiburg, Germany Nucleic acids are separated and displayed using various modifications of gel electrophoresis and detection methods. Gel electrophoresis is the core technique for genetic analysis and purification of nucleic acids for further studies. Gel …

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  • Gel Electrophoresis - Boston University

    For these purposes, a new state-of-the-art gel electrophoresis [quantitative preparative native continuous polyacrylamide gel electrophoresis proce-dure (QPNC-PAGE)] combined with biological mass and NMR spectrometries might essentially contribute to provide fundamental insights into the metabolisms of important …

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  • Important Contributions of a New Quantitative

    2017-9-30 · Quantitative proteomics of mitochondrial membrane proteins by sodium dodecyl sulphate polyacrylamide gel electrophoresis, 16O/18O stable isotope labeling and linear ion trap mass spectrometry Horacio Serrano&, Inmaculada Jorge&, Pablo Martínez-Acedo&, Pedro J. Navarro&, Daniel

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  • Quantitative proteomics of mitochondrial membrane

    1990-1-1 · We present a new method for the quantitative determination of high molecular weight alkaline phosphatase (ALP). This method consists of electrophoretic separation on polyacrylamide, incubation of the ALP isoenzymes with substrate, gel drying and quantitative evaluation by densitometric measurement.

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  • Quantitative determination of high molecular

    2019-7-1 · Microfluidic automated electrophoresis systems such as the Experion system reduce hands-on time and enable completion of protein or nucleic acid electrophoresis and analysis of up to 12 samples in a single 30–40 min assay (analysis of a single sample is accomplished in as little as 90 sec).

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  • Experion™ Automated Electrophoresis System | LSR |

    2008-12-11 · Methods: We designed an RT‐PCR strategy to amplify NPM1 exon 12 followed by electrophoresis and fragment visualization on polyacrylamide gels to discriminate a 4–5 bp size difference resulting from mutations in this gene. A hemi‐nested method was designed to increase sensitivity for the study of minimal …

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