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working principle of polyacrylamide review

  • The principle and method of polyacrylamide gel ...

    working principle of 40 nonionic polyacrylamide solution Nonionic Polyacrylamide Flocculant - Sinoloc The Nonionic Polyacrylamidecan efficiently remove all sorts of organic compounds from the acidic waste water from the beverage factory, it can help to form the floccule quicker and deposit it naturally.

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  • The principle and Procedure of Polyacrylamide Gel ...

    PAGE (Polyacrylamide Gel Electrophoresis), is an analytical method used to separate components of a protein mixture based on their size. The technique is based upon the principle that a charged molecule will migrate in an electric field towards an electrode with opposite sign.The general electrophoresis techniques cannot be used to determine the ...

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  • working principle of 40 nonionic polyacrylamide

    2015-11-17 · Principle. The concentration of polyacrylamide gels can be prepared as required in two electrophoresis systems —called “continuous system” and “discontinuous system”. The biggest feature of “discontinuous system” lies in its greatly improved sample separation resolution. Main features of this electrophoresis are: (1) Use of two gel systems with ...

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  • Polyacrylamide Gel Electrophoresis (PAGE ...

    2014-4-15 · This review discusses the principles of 2-DE as well as both recent methodological advances and new applications. Introduction Two dimensional polyacrylamide gel electrophoresis (2-DE) is considered a powerful tool used for separation and fractionation of complex protein mixtures from tissues, cells, or other biological samples.

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  • Polyacrylamide Gel Electrophoresis (Theory) :

    2011-8-1 · Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is a technique used to move charged molecules through a gel matrix by means of an electric current. This procedure is used to determine protein subunit composition, verify homogeneity of the protein sample, and purify proteins for use in other applications.

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  • Principle and Protocol of Sodium Dodecyl Sulphate ...

    2021-1-14 · Polyacrylamide conjugates of glycans have long been widely used in many research areas of glycobiology, mainly for immobilizing glycans in solid-phase assays and as multivalent inhibitors. Pending biotin tag allows immobilizing Glyc-PAA quantitatively on any surface, and acts as a tracer for detection of carbohydrate-binding proteins.

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  • Basics and recent advances of two dimensional ...

    2015-3-1 · Preparation of hydrogel based on acrylamide, acrylic acid, and its salts by inverse-suspension polymerization and diluted solution polymerization have been investigated elsewhere. Fewer studies have been done on highly concentrated solution polymerization of acrylic monomers, which are mostly patented .Chen produced acrylic acid-sodium acrylate superabsorbent through concentrated (43.6 wt ...

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  • Polyacrylamide Gel Electrophoresis (Theory) :

    PAGE is working upon the principle in which, the charged molecule will migrate towards the oppositive charged electrode through highly cross linked matrix. Separation occurs due to different rates of migration occurs by the magnitude of charge and frictional resistance related to the size. Relative Mobility: where, Z = charge on the molecule

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  • The working principle of polyacrylamide used in paper

    Polyacrylamide used in paper mills has good flocculation properties and can reduce frictional resistance between liquids. Polyacrylamide used in the papermaking industry is widely used as a retention agent, filter aid, leveling agent, water treatment agent, etc. in the papermaking field. Its function is to improve the quality of paper, improve the dewatering performance of the slurry, increase ...

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  • Basics and recent advances of two dimensional ...

    2014-4-15 · This review discusses the principles of 2-DE as well as both recent methodological advances and new applications. Introduction Two dimensional polyacrylamide gel electrophoresis (2-DE) is considered a powerful tool used for separation and fractionation of complex protein mixtures from tissues, cells, or other biological samples.

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  • SDS‐Polyacrylamide Gel Electrophoresis (SDS‐PAGE)

    2011-8-1 · Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is a technique used to move charged molecules through a gel matrix by means of an electric current. This procedure is used to determine protein subunit composition, verify homogeneity of the protein sample, and purify proteins for use in other applications.

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  • Enhanced flotation selectivity of fine coal from

    Polyacrylamide is commonly employed in selective flocculation flotation, ... According to the schematic and working principle of FBRM instrument in previous literature ... A review of induction and attachment times of wetting thin films between air bubbles and particles and its relevance in the separation of particles by flotation.

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  • SDS-PAGE- Explore the Principles, Protocols, and ...

    SDS PAGE or Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis is a technique used for the separation of proteins based on their molecular weight. It is a technique widely used in forensics, genetics, biotechnology and molecular biology to separate the protein molecules based on their electrophoretic mobility. Principle of SDS-PAGE

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  • What is meant by 'electrophoresis' ? Enumerate the

    2019-1-6 · Enumerate the working principle of sds polyacrylamide gel electrophoresis… Get the answers you need, now! jhaplu4535 jhaplu4535 29.12.2018 Biology Secondary School answered What is meant by 'electrophoresis' ? Enumerate the working principle of sds polyacrylamide gel electrophoresis with illustration. State two applications of this technique.

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  • China Pam Chemical Water Treatment Manufacturers ...

    Features. Pam Chemical Water Treatment is a linear polymer compound, and because it has a variety of active groups, it can interact with and adsorb many substances to form hydrogen bonds. It can be used in the dewatering treatment of municipal sewage, municipal sludge, papermaking sludge and …

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  • A comparative study of CE‐SDS, SDS‐PAGE, and

    2020-9-10 · What is CRISPR? CRISPR (Clusters of Regularly Interspaced Short Palindromic Repeats) technology is an emerging and powerful tool for editing genomes which allows researchers to easily alter DNA sequences and modify gene function used widely in correcting genetic defects, treating and preventing the spread of diseases and improving crops. “CRISPR” is shorthand for “CRISPR-Cas9.”

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  • Polyacrylamide Gel - an overview | ScienceDirect Topics

    Polyacrylamide gels are based on the free radical polymerization principle of acrylamide and cross-linking N,N′-methylene-bis-acrylamide. This material is physically very stable and strong. It is especially used for the electrophoretic separation of small or medium sized (up to about 1×10 6 Da) proteins. Its interaction with the migrating ...

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  • A Guide to Polyacrylamide Gel Electrophoresis and

    2018-3-5 · Polyacrylamide Gels 27 Part IV: AppendicesPolymerization 27 Percentage 28 Precast vs. Handcast 28 Format (Size and Comb Type) 29 Buffer Systems and Gel Chemistries 29 Laemmli (Tris-HCl) 31 Bis-Tris 31 Tris-Acetate 31 Tris-Tricine 31 IEF 31 Products for Handcasting Gels 32 Premade Buffers and Reagents 32 ™AnyGel Stands 32

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  • Polyacrylamide Gel Electrophoresis - an overview ...

    Polyacrylamide Gel Electrophoresis. SDS polyacrylamide gel electrophoresis shows that the 30S protein complexes of mammalian skeletal and cardiac muscles are composed of a single major high molecular weight RyR polypeptide and isoform-specific low molecular weight immunophilin (FK506 binding protein) which migrate with apparent Mr > 340000 (see Fig. 45.12B) and Mr 12000 (not visible on the ...

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  • Introduction to Polyacrylamide Gels | LSR | Bio-Rad

    2018-5-16 · Introduction. The last 25 years, and particularly the last decade, has witnessed an increased effort to develop technologies capable of identifying and quantifying large numbers of proteins expressed within a cell system (i.e., the proteome) in the hope of detecting disease biomarkers, mapping protein circuitry, or identifying novel phosphorylation sites, for example.

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  • Two-dimensional polyacrylamide gel electrophoresis

    INTRODUCTION. This protocol describes the separation of proteins by SDS-polyacrylamide gel electrophoresis. SDS is used with a reducing agent and heat to dissociate the proteins. SDS-polypeptide complexes form and migrate through the gels according to the size of the polypeptide. By using markers of known molecular weight, the molecular weight ...

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  • SDS-Polyacrylamide Gel Electrophoresis of Proteins

    2020-4-19 · Principle SDS-Polyacrylamide Gel Electrophoresis (SDS-PAGE) is probably the world’s most widely used biochemical method. In the early 60's scientists first appreciated the utility of polyacrylamide gels as a convenient and versatile Paper ID: 02015409 956.

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  • A Practical Approach on SDS PAGE for Separation of Protein

    2021-3-2 · Principle of SDS PAGE electrophoresis. The techniques polyacrylamide gel electrophoresis is a separating method of protein mixture based on molecular weight. The basic principle of SDS page electrophoresis is the separation based on molecular weight not on shape or charge of molecules.. A uniform charged molecule is migrate in an electric field towards a negative electrode (cathode) and a ...

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  • SDS PAGE Electrophoresis | Polyacrylamide Gel ...

    Review of CD4 technologies Sarah Glover Brighton and Sussex University Hospitals NHS Trust London School of Hygiene and Tropical Medicine On behalf of Members of the HIV Monitoring Technologies Working Group 15th October 2009, Geneva

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  • Review of CD4 technologies - WHO

    2019-9-23 · Guide | Scanning Electron Microscopy Working Principle 11 Sample preparation Most specimens do not need any preparation and can be directly placed into the chamber. However, if a sample contains volatile components such as water, this has to be removed beforehand. Otherwise the water would evaporate very rapidly in the evacuated environment.

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  • SDS polyacrylamide gel electrophoresis of proteins

    SDS polyacrylamide gel electrophoresis of proteins. SDS polyacrylamide gel electrophoresis of proteins Methods Mol Biol. 1994;32:23-34. doi: 10.1385/0-89603-268-X:23. ... Review MeSH terms Electrophoresis, Polyacrylamide Gel instrumentation Electrophoresis, Polyacrylamide Gel methods* ...

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  • A Practical Approach on SDS PAGE for Separation of Protein

    2020-4-19 · Principle SDS-Polyacrylamide Gel Electrophoresis (SDS-PAGE) is probably the world’s most widely used biochemical method. In the early 60's scientists first appreciated the utility of polyacrylamide gels as a convenient and versatile Paper ID: 02015409 956.

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  • Gel Electrophoresis - Boston University

    2008-9-9 · Principle of Gel Electrophoresis Agarose Gel Electrophoresis Polyacrylamide Gel Electrophoresis doi: 10.1038/npg.els.0005335 Gel Electrophoresis ENCYCLOPEDIA OF LIFE SCIENCES & 2005, John Wiley & Sons, Ltd. www.els.net 1

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  • Overview of Electrophoresis | Thermo Fisher Scientific

    Polyacrylamide gel electrophoresis in progress. Prepared gel cassettes are inserted into a gel tank, in this case the Invitrogen Mini Gel Tank, which holds two mini gels at a time.After wells are loaded with protein samples, the gels submerged in a conducting running buffer, and electrical current is applied, typically for 20 to 40 minutes.

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  • Lecture 13 Electrophoresis (Part-I)

    2017-8-4 · Principle of the technique: In the discontinuous system, negatively charged detergent, sodium dodecyl sulphate (SDS), is used to denature protein and impart a ... There are additional accessories needed for casting the polyacrylamide gel such as comb (to prepare different well), spacer, gel caster etc.

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  • Review of CD4 technologies - WHO

    Review of CD4 technologies Sarah Glover Brighton and Sussex University Hospitals NHS Trust London School of Hygiene and Tropical Medicine On behalf of Members of the HIV Monitoring Technologies Working Group 15th October 2009, Geneva

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  • A Review on the Working Principles of a Vacuum

    2019-12-13 · A Review on the Working Principles of a Vacuum Furnace. As a rule, the inner workings of a vacuum furnace are ordinarily composed of a series of sealed firebox chambers. At the centre of this airtight assemblage, a leakproof furnace shell is hooked to an air purging pump. Heat-inducing elements, perhaps made out of fitted graphite or ceramic ...

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  • A Guide to Polyacrylamide Gel Electrophoresis and

    2018-3-5 · Polyacrylamide Gels 27 Part IV: AppendicesPolymerization 27 Percentage 28 Precast vs. Handcast 28 Format (Size and Comb Type) 29 Buffer Systems and Gel Chemistries 29 Laemmli (Tris-HCl) 31 Bis-Tris 31 Tris-Acetate 31 Tris-Tricine 31 IEF 31 Products for Handcasting Gels 32 Premade Buffers and Reagents 32 ™AnyGel Stands 32

    Get Price
  • A Practical Approach on SDS PAGE for Separation of

    2020-4-19 · Principle SDS-Polyacrylamide Gel Electrophoresis (SDS-PAGE) is probably the world’s most widely used biochemical method. In the early 60's scientists first appreciated the utility of polyacrylamide gels as a convenient and versatile Paper ID: 02015409 956.

    Get Price
  • Lecture 13 Electrophoresis (Part-I)

    2017-8-4 · Principle of the technique: In the discontinuous system, negatively charged detergent, sodium dodecyl sulphate (SDS), is used to denature protein and impart a ... There are additional accessories needed for casting the polyacrylamide gel such as comb (to prepare different well), spacer, gel caster etc.

    Get Price
  • SDS polyacrylamide gel electrophoresis of proteins

    SDS polyacrylamide gel electrophoresis of proteins. SDS polyacrylamide gel electrophoresis of proteins Methods Mol Biol. 1994;32:23-34. doi: 10.1385/0-89603-268-X:23. ... Review MeSH terms Electrophoresis, Polyacrylamide Gel instrumentation Electrophoresis, Polyacrylamide Gel methods* ...

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  • Protocol Online: Denaturing Urea-Polyacrylamide Gel ...

    2019-12-20 · Principle Polyacrylamide gels are chemically cross-linked gels forming by the polymerization of acrylamide with a cross-linking agent, usually N, N’-methylene bisacrylamide (Bis). The polymerization initiates by free radical formation usually carrying out with ammonium per sulfate as the initiator and N, N, N’, N’-tetramethylene diamine ...

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  • Native PAGE - Molbio

    2016-8-9 · Caution:Always wear gloves, safety glasses, and a surgical mask when working withacrylamide powder since it is a neurotoxin. Commerciallyprepared polyacrylamide solutions (National Diagnostics) are availableand highly recommended since they have long shelf lives and do not involvemassing the neurotoxic acrylamide powder.

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  • Copolymer Polyacrylamide Water Treatment Of

    2. Sewage Water Treatment. For sewage water solid/liquid separation, if matched with PAC or aluminum sulphate, the effect will be excellent, also used for sludge dewatering. 3. Industrial Wastewater Treatment. Dosing polyacrylamide to removal suspended solids, and can reduce the consumption of coagulant and speed up sedimentation.

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  • A Practical Approach on SDS PAGE for Separation of

    2020-4-19 · Principle SDS-Polyacrylamide Gel Electrophoresis (SDS-PAGE) is probably the world’s most widely used biochemical method. In the early 60's scientists first appreciated the utility of polyacrylamide gels as a convenient and versatile Paper ID: 02015409 956.

    Get Price
  • Lecture 13 Electrophoresis (Part-I)

    2017-8-4 · Principle of the technique: In the discontinuous system, negatively charged detergent, sodium dodecyl sulphate (SDS), is used to denature protein and impart a ... There are additional accessories needed for casting the polyacrylamide gel such as comb (to prepare different well), spacer, gel caster etc.

    Get Price
  • Running agarose and polyacrylamide gels

    2011-6-17 · The basics. Agarose gels can be used to resolve large fragments of DNA. Polyacrylamide gels are used to separate shorter nucleic acids, generally in the range of 1−1000 base pairs, based on the concentration used (Figure 1). These gels can be run with or without a denaturant. Gels that are run without a denaturant are referred to as native gels.

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  • SDS polyacrylamide gel electrophoresis of proteins

    SDS polyacrylamide gel electrophoresis of proteins. SDS polyacrylamide gel electrophoresis of proteins Methods Mol Biol. 1994;32:23-34. doi: 10.1385/0-89603-268-X:23. ... Review MeSH terms Electrophoresis, Polyacrylamide Gel instrumentation Electrophoresis, Polyacrylamide Gel methods* ...

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  • SDS Page - Principle, Functions, Protocol, Applications ...

    2020-8-1 · SDS PAGE also known as Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis is a technique used for separating the proteins based on their molecular weight. It is a widely used technique in forensics, genetics, biotechnology, and molecular biology to separate the protein molecules based on their electrophoretic mobility. Principle of SDS-PAGE

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  • polyacrylamide for sludge treatment, polyacrylamide

    Polyacrylamide in waste water treatment sludge. Polyacrylamide in waste water treatment sludge dewatering processing. Main Production: 1. Oilfield Chemicals, Oilfield chemical is a general germ,The oilfield chemicals we can supply include anionic polyacrylamide used for oil drilling mud additives,drag reduction agent and EOR(enhanced oil recovery)agent .

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  • Types of Electrophoresis - Principles and Applications ...

    2021-5-27 · Types of Electrophoresis – Principles and Applications. Electrophoresis is a process that separates charged particles in a fluid with the aid of the field of electrical charge. In life sciences, an electrophoresis is a vital tool that separates protein molecules or DNA according to its size and type. In a laboratory setting, electrophoresis ...

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  • Muscle-fiber array inspired, multiple-mode, pneumatic ...

    2021-3-24 · (E) Working principle of the one-step fabrication approach for rolling the passive 2D elastomer membrane into a 3D MAIPAM (Movie S1). Here, we present a class of active 3D muscle-fiber array inspired pneumatic artificial muscles (termed muscle-fiber array inspired pneumatic artificial muscles (MAIPAMs), Fig. 1 B) that demonstrate the ability of ...

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  • Four Principles to Ensure Hybrid Work Is Productive

    2019-12-13 · A Review on the Working Principles of a Vacuum Furnace. As a rule, the inner workings of a vacuum furnace are ordinarily composed of a series of sealed firebox chambers. At the centre of this airtight assemblage, a leakproof furnace shell is hooked to an air purging pump. Heat-inducing elements, perhaps made out of fitted graphite or ceramic ...

    Get Price
  • Four Principles to Ensure Hybrid Work Is Productive

    2011-8-1 · Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is a technique used to move charged molecules through a gel matrix by means of an electric current. This procedure is used to determine protein subunit composition, verify homogeneity of the protein sample, and purify proteins for use in other applications.

    Get Price
  • SDS‐Polyacrylamide Gel Electrophoresis (SDS‐PAGE)

    2011-6-17 · The basics. Agarose gels can be used to resolve large fragments of DNA. Polyacrylamide gels are used to separate shorter nucleic acids, generally in the range of 1−1000 base pairs, based on the concentration used (Figure 1). These gels can be run with or without a denaturant. Gels that are run without a denaturant are referred to as native gels.

    Get Price
  • Running agarose and polyacrylamide gels

    2019-12-13 · A Review on the Working Principles of a Vacuum Furnace. As a rule, the inner workings of a vacuum furnace are ordinarily composed of a series of sealed firebox chambers. At the centre of this airtight assemblage, a leakproof furnace shell is hooked to an air purging pump. Heat-inducing elements, perhaps made out of fitted graphite or ceramic ...

    Get Price
  • A Review on the Working Principles of a Vacuum

    2017-8-4 · Principle of the technique: In the discontinuous system, negatively charged detergent, sodium dodecyl sulphate (SDS), is used to denature protein and impart a ... There are additional accessories needed for casting the polyacrylamide gel such as comb (to prepare different well), spacer, gel caster etc.

    Get Price
  • Lecture 13 Electrophoresis (Part-I)

    2020-8-1 · SDS PAGE also known as Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis is a technique used for separating the proteins based on their molecular weight. It is a widely used technique in forensics, genetics, biotechnology, and molecular biology to separate the protein molecules based on their electrophoretic mobility. Principle of SDS-PAGE

    Get Price
  • SDS Page - Principle, Functions, Protocol, Applications ...

    In this study, the flocculation performance and kinetics of magnetic cationic polyacrylamide (MCPAM) microspheres, compared with cationic polyacrylamide (CPAM), were systematically investigated under different magnetic field strengths. Flocculation performance was observed by jar test experiment. The density of flocs was estimated by the determination of floc settlement velocity and image ...

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  • Flocculation Performance and Kinetics of Magnetic ...

    2018-6-20 · The Principle And Method Of Polyacrylamide Gel Electropsis Recipe for polyacrylamide gel recipe for polyacrylamide gel sds western webpage powered by ramaz geguchadze recipe for polyacrylamide gel. Whats people lookup in this blog: Denaturing Polyacrylamide Gel Recipe; Non Denaturing Polyacrylamide Gel Recipe; Non Denaturing Page Gel Recipe

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